Burrin D G, Wang H, Heath J, Dudley M A
Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA.
Pediatr Res. 1996 Jul;40(1):72-6. doi: 10.1203/00006450-199607000-00013.
Lactoferrin is a polypeptide which is abundant in colostrum; however, its biologic effect in the neonate is unknown. The objective was to determine the potentially anabolic effect of orally administered lactoferrin on visceral organ growth and protein synthesis in newborn pigs. We studied a total of 18 unsuckled newborn pigs from six litters. Three pigs from each litter were randomly assigned to one of three dietary treatment groups (n = 6) and bottle-fed (10 mL/h) formula, formula containing physiologic levels (1 mg/mL) of added bovine lactoferrin (bLF), or colostrum. After 24 h of feeding, we measured visceral organ protein synthesis in vivo using a flooding dose of [3H]phenylalanine. We also measured visceral organ protein and DNA mass, as well as intestinal hydrolase activities and villus morphology. Hepatic protein synthesis in pigs fed either formula containing bLF or colostrum was similar and in both groups was significantly higher than in pigs fed formula. Splenic protein synthesis was not significantly different in pigs fed either formula or formula containing bLF, but was significantly higher in colostrum-fed animals. There were no significant differences in small intestinal growth, protein synthesis, or hydrolase activities between newborn pigs fed formula, formula containing bLF, or colostrum. Our results demonstrate that feeding formula containing physiologic concentrations of added bLF increased hepatic protein synthesis in newborn pigs, suggesting that colostrumborne lactoferrin serves an anabolic function in neonates.
乳铁蛋白是一种在初乳中含量丰富的多肽；然而，其对新生儿的生物学作用尚不清楚。目的是确定口服乳铁蛋白对新生猪内脏器官生长和蛋白质合成的潜在合成代谢作用。我们总共研究了来自6窝的18头未吮乳的新生猪。每窝中的3头猪被随机分配到三个饮食处理组之一（n = 6），并用奶瓶喂养（10 mL/h）配方奶、含有生理水平（1 mg/mL）添加牛乳铁蛋白（bLF）的配方奶或初乳。喂食24小时后，我们使用大剂量的[3H]苯丙氨酸体内测量内脏器官蛋白质合成。我们还测量了内脏器官蛋白质和DNA质量，以及肠道水解酶活性和绒毛形态。喂食含有bLF的配方奶或初乳的猪的肝脏蛋白质合成相似，且两组均显著高于喂食配方奶的猪。喂食配方奶或含有bLF的配方奶的猪的脾脏蛋白质合成没有显著差异，但喂食初乳的动物的脾脏蛋白质合成显著更高。喂食配方奶、含有bLF的配方奶或初乳的新生猪在小肠生长、蛋白质合成或水解酶活性方面没有显著差异。我们的结果表明，喂食含有生理浓度添加bLF的配方奶可增加新生猪的肝脏蛋白质合成，这表明初乳中的乳铁蛋白在新生儿中发挥合成代谢功能。
