Rito-Palomares M, Lyddiatt A
BBSRC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, UK.
J Chromatogr B Biomed Appl. 1996 May 17;680(1-2):81-9. doi: 10.1016/0378-4347(95)00500-5.
A practical study is presented of the influence of cell debris and polymer recycling upon the operation of two-stage aqueous two-phase systems (ATPS) for the recovery of yeast bulk protein, pyruvate kinase and fumarase. Brewers' yeast was disrupted using one of two types of high-pressure homogenisers or a bead mill. The different cell debris suspensions were partitioned in a single PEG-phosphate ATPS extraction and the efficiency of solid-liquid separation was examined. A continuously operated two-stage ATPS process, using spray columns, is presented and practical problems of polymer recycling are discussed. Conclusions are drawn concerning the generic implementation and operational stability of ATPS in practical protein recoveries.
本文介绍了一项关于细胞碎片和聚合物循环利用对用于回收酵母总蛋白、丙酮酸激酶和延胡索酸酶的两级水相两相系统（ATPS）运行影响的实际研究。使用两种高压均质机之一或珠磨机破碎啤酒酵母。将不同的细胞碎片悬浮液在单一的聚乙二醇-磷酸盐ATPS萃取中进行分配，并考察固液分离效率。介绍了一种使用喷雾柱的连续运行两级ATPS工艺，并讨论了聚合物循环利用的实际问题。得出了关于ATPS在实际蛋白质回收中的一般实施和操作稳定性的结论。
