Novotná J, Deyl Z, Miksík I
Department of Medical Chemistry and Biochemistry, Charles University, 2nd Medical School, Prague, Czech Republic.
J Chromatogr B Biomed Appl. 1996 May 31;681(1):77-82. doi: 10.1016/0378-4347(96)00014-x.
Collagen type-I CNBr peptides were separated under acidic conditions by capillary electrophoresis. Separation conditions were: 100 mM phosphate buffer pH 2.5, 50 cm x 50 microns capillary (placed in a cartridge), 8 kV, running time 30-45 min, detection by UV at 200 nm. The peptides were separated strictly by their molecular mass and the overall pattern was well comparable to RP-HPLC separations of these analytes. It is proposed that the separation mechanism may involve hydrophobic sorptions to the capillary wall.
I型胶原蛋白溴化氰肽在酸性条件下通过毛细管电泳进行分离。分离条件为：100 mM pH 2.5的磷酸盐缓冲液，50 cm×50微米毛细管（置于柱盒中），8 kV，运行时间30 - 45分钟，在200 nm处通过紫外检测。这些肽严格按照分子量进行分离，整体图谱与这些分析物的反相高效液相色谱分离结果具有良好的可比性。有人提出，分离机制可能涉及肽与毛细管壁的疏水吸附作用。
