Onaolapo J A, Salami J O
Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria.
Afr J Med Med Sci. 1995 Sep;24(3):275-81.
The effect of subminimum inhibitory concentration (subMIC) of ceftriaxone on adherence of two isolates of Pseudomonas aeruginosa onto inert surfaces (catheter, plastic and glass) was studied. It was found that the phase of growth of Ps. aeruginosa and the nature of the inert surfaces affected the adherence. One-twenty fourth of the M.I.C. increased the adherence of the clinical isolate in the exponential phase of growth but decreased it during the stationary phase; the reverse was the case for the wild type isolate. When the inert surfaces were coated with serum, the adherence of the clinical isolate also increased during the exponential phase of growth, while that of the wild type increased in the stationary phase. Changes in the surface properties of the test organisms indicated that the subMIC of Ceftriaxone mediated increase in hydrophobicity at both phases of growth. These results suggest that sub-inhibitory levels of Ceftriaxone may decrease the virulence of P. aeruginosa since a good polymorphnuclear leucocytebacterium contact will result in the bacterium being strongly phagocytosed because adherence has also been implicated in the process of phagocytosis.
研究了头孢曲松亚最小抑菌浓度（subMIC）对两株铜绿假单胞菌在惰性表面（导管、塑料和玻璃）上黏附的影响。发现铜绿假单胞菌的生长阶段和惰性表面的性质会影响黏附。临床分离株在生长指数期，1/24的最低抑菌浓度（M.I.C.）增加了其黏附，但在稳定期则降低了黏附；野生型分离株的情况则相反。当惰性表面涂有血清时，临床分离株在生长指数期的黏附也增加，而野生型在稳定期增加。受试微生物表面性质的变化表明，头孢曲松的亚MIC在两个生长阶段均介导了疏水性的增加。这些结果表明，头孢曲松的亚抑制水平可能会降低铜绿假单胞菌的毒力，因为良好的多形核白细胞-细菌接触会导致细菌被强烈吞噬，因为黏附也与吞噬过程有关。
