Verrotti A C, Thompson S R, Wreden C, Strickland S, Wickens M
Department of Molecular Pharmacology, University Medical Center at Stony Brook, NY 11794, USA.
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9027-32. doi: 10.1073/pnas.93.17.9027.
Cytoplasmic polyadenylylation is an evolutionarily conserved mechanism involved in the translational activation of a set of maternal messenger RNAs (mRNAs) during early development. In this report, we show by interspecies injections that Xenopus and mouse use the same regulatory sequences to control cytoplasmic poly(A) addition during meiotic maturation. Similarly, Xenopus and Drosophila embryos exploit functionally conserved signals to regulate polyadenylylation during early post-fertilization development. These experiments demonstrate that the sequence elements that govern cytoplasmic polyadenylylation, and hence one form of translational activation, function across species. We infer that the requisite regulatory sequence elements, and likely the trans-acting components with which they interact, have been conserved since the divergence of vertebrates and arthropods.
胞质多聚腺苷酸化是一种进化上保守的机制，参与早期发育过程中一组母源信使核糖核酸（mRNA）的翻译激活。在本报告中，我们通过种间注射表明，非洲爪蟾和小鼠在减数分裂成熟过程中使用相同的调控序列来控制胞质多聚腺苷酸的添加。同样，非洲爪蟾和果蝇胚胎在受精后早期发育过程中利用功能保守的信号来调节多聚腺苷酸化。这些实验表明，控制胞质多聚腺苷酸化的序列元件，以及因此一种翻译激活形式，在物种间发挥作用。我们推断，自脊椎动物和节肢动物分化以来，必需的调控序列元件以及可能与之相互作用的反式作用成分一直保守存在。
