Brand W A
Finnigan MAT, Bremen, Germany.
J Mass Spectrom. 1996 Mar;31(3):225-35. doi: 10.1002/(SICI)1096-9888(199603)31:3<225::AID-JMS319>3.0.CO;2-L.
Isotope ratio monitoring following on-line combustion is a new method in gas chromatography/mass spectrometry (GC/MS) that allows the ratios of abundances of stable isotopes of elements such as carbon and nitrogen to be determined for individual compounds introduced via a gas chromatograph. It is the first combustion method that allows direct measurement of isotope ratios of individual molecular components of mixtures. Unlike traditional gas isotope ratio measurements made on pure samples, the method requires only very small samples, viz. several picolitres of the vapour. This paper reviews the principles and history of gas isotope ratio monitoring, focusing on the instrumentation used in isotope ratio monitoring GC/MS, including the combustion procedures used for atomization. Standardization methods and data manipulation techniques are described, as are applications to geochemistry, biology, medicine and other areas of science.
在线燃烧后的同位素比率监测是气相色谱/质谱联用仪（GC/MS）中的一种新方法，它能够测定通过气相色谱仪引入的各个化合物中碳、氮等元素稳定同位素的丰度比。这是第一种能够直接测量混合物中各个分子成分同位素比率的燃烧方法。与对纯样品进行的传统气体同位素比率测量不同，该方法仅需要非常少量的样品，即几皮升的蒸汽。本文综述了气体同位素比率监测的原理和历史，重点介绍了同位素比率监测GC/MS中使用的仪器，包括用于原子化的燃烧程序。文中还描述了标准化方法和数据处理技术，以及在地球化学、生物学、医学和其他科学领域的应用。
