Milcent M C, Goudard F, Durand J P, Germain P, Pieri J, George S G
Laboratoire de Biochimie et Radiobiochimie, GERMETRAD, Université de Nantes, France.
Biochem Mol Biol Int. 1996 May;39(1):137-48. doi: 10.1080/15216549600201141.
The subcellular distribution and binding of 241Am and 137Cs in the visceral mass of the oyster Crassostrea gigas were investigated following exposure to sea water contaminated with these radionuclides. 241Am was predominantly sequestered by the lysosomal system. Approximately, 10% of 241Am was associated with soluble macromolecules. 241Am was bound to lipofuscin, ferritin and to unidentified ligands of 60 to 15 kdaltons mol. wt. No evidence was found for binding of 241Am to metallothionein synthesized de novo. In contrast, only small amounts of 137Cs were present in lysosomes and 137Cs was not associated with soluble cellular proteins. These results indicate that they enter complete separate metabolic pathways.
在将牡蛎巨蛎暴露于被这些放射性核素污染的海水中之后，对241Am和137Cs在其内脏团中的亚细胞分布及结合情况进行了研究。241Am主要被溶酶体系统隔离。大约10%的241Am与可溶性大分子相关联。241Am与脂褐素、铁蛋白以及分子量为60至15千道尔顿的未鉴定配体相结合。未发现有证据表明241Am与新合成的金属硫蛋白相结合。相比之下，溶酶体中仅存在少量的137Cs，且137Cs与可溶性细胞蛋白无关。这些结果表明它们进入了完全不同的代谢途径。
