Xie W S, Yuc J C, Tu Y P
National Laboratory of Biomacromolecules, Academia Sinica, Beijing, China.
Biochem Mol Biol Int. 1996 May;39(2):279-84. doi: 10.1080/15216549600201291.
The nanosecond time-resolved fluorescence and fluorescence lifetime quenching have been used to detect the conformational changes of human erythrocyte glucose transporter induced by its ligands. Results show that D-glucose can decrease the fluorescence lifetimes, while L-glucose exhibits no effect. The fluorescence lifetime quenching results also show that in the presence of D-glucose, the quenching efficiency of hypocrellin B (a hydrophobic quencher obtained from a parasitic fungus, growing in Yunnan, China) is lower than in the presence of L-glucose. It can be deduced that the conformational changes of human erythrocyte glucose transporter induced by D-glucose are different from those induced by L-glucose.
纳秒时间分辨荧光和荧光寿命猝灭已被用于检测人红细胞葡萄糖转运蛋白受其配体诱导的构象变化。结果表明，D - 葡萄糖可降低荧光寿命，而L - 葡萄糖则无此作用。荧光寿命猝灭结果还表明，在D - 葡萄糖存在下，竹红菌素B（一种从生长于中国云南的寄生真菌中获得的疏水性猝灭剂）的猝灭效率低于在L - 葡萄糖存在下的猝灭效率。由此可以推断，D - 葡萄糖诱导的人红细胞葡萄糖转运蛋白的构象变化与L - 葡萄糖诱导的不同。
