Glück A, Wool I G
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA.
Biochem Mol Biol Int. 1996 May;39(2):285-91. doi: 10.1080/15216549600201301.
Ricin A-chain is a cytotoxic RNA N-glycosidase that inactivates eukaryotic ribosomes by depurinating the adenosine at position 4324 in 28S rRNA. The enzyme retains its specificity when a synthetic oligoribonucleotide (a 35-mer) that mimics the structure at the site of action is the substrate. However, covalent modification by ricin A-chain of the oligoribonucleotide but not of ribosomes, depends on the simultaneous presence of a divalent cation and a chelating agent.
蓖麻毒素A链是一种细胞毒性RNA N-糖苷酶，它通过使28S rRNA中第4324位的腺苷脱嘌呤来使真核核糖体失活。当模拟作用位点结构的合成寡核糖核苷酸（35聚体）作为底物时，该酶保持其特异性。然而，蓖麻毒素A链对寡核糖核苷酸而非核糖体的共价修饰取决于二价阳离子和螯合剂的同时存在。
