鳗鱼肝脏线粒体中三羧酸载体的部分纯化与重组

Partial purification and reconstitution of the tricarboxylate carrier from eel liver mitochondria.

作者信息

Zara V, Damiano G, Franco M R, Siculella L, Gnoni G V

机构信息

Dept. of Biology, University of Lecce, Italy.

出版信息

Biochem Mol Biol Int. 1996 May;39(2):369-75. doi: 10.1080/15216549600201401.

DOI:10.1080/15216549600201401

PMID:8799465

Abstract

The tricarboxylate carrier of the inner membrane of eel liver mitochondria has been solubilized with Triton X-100 and partially purified by chromatography of the mitochondrial extract on dry hydroxyapatite. The purified fraction has been reconstituted into liposomes and functionally analyzed. The reconstituted carrier protein catalyzed a 1,2,3-benzenetricarboxylate-sensitive citrate uptake in liposomes. The substrate specificity and the inhibitor sensitivity of the tricarboxylate carrier are similar to those previously determined for the same protein of mammalian mitochondria.

摘要

鳗鱼肝脏线粒体内膜的三羧酸载体已用 Triton X - 100 增溶，并通过将线粒体提取物在干羟基磷灰石上进行色谱分析进行了部分纯化。纯化后的组分已被重组成脂质体并进行了功能分析。重组后的载体蛋白催化脂质体中对 1,2,3 - 苯三羧酸敏感的柠檬酸摄取。三羧酸载体的底物特异性和抑制剂敏感性与先前对哺乳动物线粒体相同蛋白所确定的相似。