Stewart M J, Emery D L, McClure S J, Bendixsen T
CSIRO Division of Animal Production, McMaster Laboratory, Blacktown, NSW, Australia.
Immunol Cell Biol. 1996 Jun;74(3):255-7. doi: 10.1038/icb.1996.45.
Isolated mucosal mast cells (MMC) were used to examine the ability of four neuropeptides, substance P, vasoactive intestinal peptide, beta-endorphin and somatostatin, to release mediators in the presence or absence of parasite antigen. None of the neuropeptides induced the release of sheep mast cell protease (SMCP) or histamine from MMC of helminth-immune sheep in the absence of parasite antigen. Incubation of immune MMC with 100 and 1.0 microgram/mL parasite antigen induced 32.1 and 15.5% specific SMCP release, respectively. While the neuropeptides did not augment SMCP release at 100 micrograms/mL parasite antigen, significant enhancement (40-98%) of SMCP release at 1 microgram/mL antigen was obtained by each neuropeptide at concentrations from 10(-8) to 10(-12) mol/L. The results provide additional support for modulation of MMC degranulation by neural activity in sheep and, to our knowledge, this is the first demonstration that the threshold antigen concentration for allergic responses may also be lowered by neuropeptides to render the reaction more sensitive to antigen.
分离出的黏膜肥大细胞（MMC）用于检测四种神经肽，即P物质、血管活性肠肽、β-内啡肽和生长抑素，在有或无寄生虫抗原存在的情况下释放介质的能力。在没有寄生虫抗原的情况下，这些神经肽均未诱导来自蠕虫免疫绵羊的MMC释放绵羊肥大细胞蛋白酶（SMCP）或组胺。用100和1.0微克/毫升寄生虫抗原孵育免疫MMC，分别诱导32.1%和15.5%的特异性SMCP释放。虽然神经肽在100微克/毫升寄生虫抗原时不会增加SMCP释放，但每种神经肽在浓度为10^(-8)至10^(-12)摩尔/升时，在1微克/毫升抗原时可显著增强（40 - 98%）SMCP释放。这些结果为绵羊中神经活动对MMC脱颗粒的调节提供了额外支持，据我们所知，这是首次证明神经肽也可降低过敏反应的阈值抗原浓度，使反应对抗原更敏感。
