外周接种诱导的抗原特异性分泌 IgA 细胞

Antigen-specific IgA-secreting cells induced by peripheral vaccination.

作者信息

Rothel J S, Corner L A, Seow H F, Wood P R, Lightowlers M W

机构信息

University of Melbourne, Molecular Parasitology Laboratory, Werribee, Victoria, Australia.

出版信息

Immunol Cell Biol. 1996 Jun;74(3):298-300. doi: 10.1038/icb.1996.52.

DOI:10.1038/icb.1996.52

PMID:8799732

Abstract

Antigen-specific IgA-secreting cells were shown to be generated in peripheral lymph nodes following s.c. vaccination. The efferent duct of prefemoral lymph nodes of sheep were cannulated prior to vaccination at a site draining to the cannulated node. Vaccines were contained a recombinant protein (Taenia ovis 45W-GST) and either incomplete Freund's, Quil A or Al(OH)3 as adjuvant. Lymph fluid was examined for the presence of 45W-GST-specific antibody by ELISA and antibody-secreting cells by enzyme-linked immunospot (ELISPOT) assay. Large numbers of anti-45W-GST IgA-secreting cells were detected at various times after vaccination as were IgM, IgG1 and IgG2-secreting cells. For sheep vaccinated using incomplete Freund's adjuvant, up to 11% (> 1 million/day) of 45W-GST-specific ELISPOT were IgA-secreting cells.

摘要

抗原特异性分泌 IgA 细胞在皮下接种疫苗后于外周淋巴结中产生。在接种疫苗前，将绵羊股前淋巴结的输出管插管至引流至插管淋巴结的部位。疫苗包含重组蛋白（绵羊带绦虫 45W - GST）以及弗氏不完全佐剂、Quil A 或氢氧化铝作为佐剂。通过 ELISA 检测淋巴液中 45W - GST 特异性抗体的存在情况，并通过酶联免疫斑点（ELISPOT）测定法检测抗体分泌细胞。接种疫苗后的不同时间均检测到大量分泌抗 45W - GST IgA 的细胞，以及分泌 IgM、IgG1 和 IgG2 的细胞。对于使用弗氏不完全佐剂接种的绵羊，高达 11%（> 100 万/天）的 45W - GST 特异性 ELISPOT 是分泌 IgA 的细胞。