Spencer T E, Ing N H, Ott T L, Mayes J S, Becker W C, Watson G H, Mirando M A, Brazer F W
Department of Animal Science, Texas A&M University, College Station 77843-2471, USA.
J Mol Endocrinol. 1995 Oct;15(2):203-20. doi: 10.1677/jme.0.0150203.
This study determined the effects of intrauterine injections of recombinant ovine interferon-tau; (roIFN-tau; 2 x 10(7) antiviral units/day) or control proteins (6 mg/day) from day 11 to day 14 post-oestrus = day 0) on endometrial expression of receptors fro oestrogen, progesterone and oxytocin in cyclic ewes. Plasma concentrations of progesterone were greater on day 15 in ewes receiving roIFN-tau compared with control proteins (P < 0.02, treatment x day). Ewes injected with roIFN-tau had lower endometrial levels or oestrogen receptor mRNA (P > 0.10) and protein (P < 0.01) on day 15 compared with ewes receiving control proteins. In situ hybridization analysis indicated that oestrogen receptor mRNA was more abundant in the luminal and glandular epithelium of control ewes compared with roIFN-tau-treated ewes. Immunoreactive oestrogen receptor was also present in the luminal and glandular epithelium of control, but not roIFN-tau-treated ewes. Endometrial levels of progesterone receptor mRNA and protein were not different (P > 0.10) between control and roIFN-tau-treated ewes. In situ hybridization analyses indicated that progesterone receptor mRNA abundance was low in endometrial epithelium and stroma of both control and roIFN-tau-injected ewes. Immunoreactive progesterone receptors were present in the endometrial stroma and epithelium of control ewes, but confined to the stroma of roIFN-tau-treated ewes. Oxytocin receptor density was lower (P < 0.01) in the endometrium of ewes injected with roIFN-tau than control proteins; however, oxytocin receptor affinity was not affected (P > 0.10) by treatment. Concentrations of 13,14-dihydro-15-ketoprostaglandin F2a (PGFM) were not increased by exogenous oxytocin administration in control and roIFN-tau-treated ewes on days 10 or 12 post-oestrus. However, on day 14, control ewes responded to oxytocin with increased plasma concentrations of PGFM, whereas ewes receiving roIFN-tau remained unresponsive to oxytocin. These results indicate that the an tiluteolytic effects of IFN-tau are to prevent increases in endometrial oestrogen receptor MRNA and protein and oxytocin receptor density which abrogates uterine release of prostaglandin F2a during maternal recognition of pregnancy. IFN-tau may inhibit the synthesis of oestrogen receptor mRNA by a transcriptional or post-transcriptional regulatory mechanism to suppress oxytocin receptor formation during early pregnancy in ewes.
本研究确定了在发情后期第11天至第14天（发情日=第0天）向子宫内注射重组羊干扰素τ（roIFN-τ；2×10⁷抗病毒单位/天）或对照蛋白（6毫克/天）对周期性母羊子宫内膜雌激素、孕激素和催产素受体表达的影响。与注射对照蛋白的母羊相比，接受roIFN-τ的母羊在第15天的血浆孕酮浓度更高（P<0.02，处理×天数）。与接受对照蛋白的母羊相比，注射roIFN-τ的母羊在第15天的子宫内膜雌激素受体mRNA水平（P>0.10）较低，而蛋白水平（P<0.01）较低。原位杂交分析表明，与roIFN-τ处理的母羊相比，对照母羊的腔上皮和腺上皮中雌激素受体mRNA更丰富。免疫反应性雌激素受体也存在于对照母羊的腔上皮和腺上皮中，但不存在于roIFN-τ处理的母羊中。对照母羊和roIFN-τ处理的母羊之间的子宫内膜孕激素受体mRNA和蛋白水平没有差异（P>0.10）。原位杂交分析表明，对照母羊和注射roIFN-τ的母羊的子宫内膜上皮和基质中孕激素受体mRNA丰度均较低。免疫反应性孕激素受体存在于对照母羊的子宫内膜基质和上皮中，但仅限于roIFN-τ处理母羊的基质中。注射roIFN-τ的母羊子宫内膜中的催产素受体密度低于对照蛋白处理的母羊（P<0.01）；然而，处理对催产素受体亲和力没有影响（P>0.10）。在发情后期第10天或第12天，对照母羊和roIFN-τ处理的母羊给予外源性催产素后，13,14-二氢-15-酮前列腺素F2α（PGFM）的浓度没有增加。然而，在第14天，对照母羊对催产素的反应是血浆PGFM浓度升高，而接受roIFN-τ的母羊对催产素仍无反应。这些结果表明，IFN-τ的抗溶解黄体作用是防止子宫内膜雌激素受体mRNA和蛋白以及催产素受体密度增加，从而在母羊妊娠识别期间消除子宫前列腺素F2α的释放。IFN-τ可能通过转录或转录后调节机制抑制雌激素受体mRNA的合成，从而在母羊妊娠早期抑制催产素受体的形成。
