Hasegawa S, Ritter J H, Patterson A, Ockner D M, Sawa H, Mohanakumar T, Cooper J D, Wick M R
Division of Cardiothoracic Surgery, Washington University Medical Center, St. Louis, Mo 63110, USA.
J Heart Lung Transplant. 1995 Sep-Oct;14(5):897-905.
The expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and class II major histocompatibility complex antigens was studied in control lung tissue and preserved human donor lungs. The three controls were represented by wedge biopsy specimens taken from non-neoplastic lung surrounding bronchogenic carcinomas.
Nine lungs were harvested from six brain-dead donors, flushed with Euro-Collins solution or low potassium-dextran-glucose solution, and stored at 1 degree C or 10 degrees C. Samples of the latter organs were taken at the time of surgical harvest (baseline) and after 2, 12, 24, and 48 hours of preservation time. Immunostains with monoclonal antibodies against intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and class II major histocompatibility complex molecules were performed on all samples, and the relative presence of these determinants was evaluated.
In both the controls and preserved lungs, intercellular adhesion molecule-1 expression was intense in the septal capillary endothelium and alveolar pneumocytes, but essentially absent in bronchial epithelium. Vascular cell adhesion molecule-1 was moderately to strongly labeled in the endothelia of large and small blood vessels of all types, and it was not seen in other cell types. Class II major histocompatibility complex antigens were variably observed in pulmonary epithelial cells, but they were not expressed by endothelia. There appeared to be no significant difference in the immunohistologic density of intercellular adhesion molecule-1 or vascular cell adhesion molecule-1 immunostaining in allografts at the specified time points of preservation; this conclusion was confirmed by Western blot analysis. Similar findings pertained to staining results for human leukocyte DR antigens. There was likewise no significant difference in the expression of the three analytes when donor lungs perfused with Euro-Collins solution versus low potassium-dextran-glucose solution were compared; this was also true of organs preserved at 1 degree C versus 10 degrees C.
These results suggest that, in the immediate post-harvest period, modulations in the expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or class II major histocompatibility complex antigens in pulmonary allografts are not attributable to the influences of preservation conditions.
研究了细胞间黏附分子-1、血管细胞黏附分子-1和II类主要组织相容性复合体抗原在对照肺组织和保存的人供体肺中的表达情况。三个对照组由取自支气管源性癌周围非肿瘤性肺组织的楔形活检标本代表。
从6名脑死亡供体获取9个肺，用Euro-Collins溶液或低钾右旋糖酐葡萄糖溶液冲洗，并在1℃或10℃保存。在手术获取时（基线）以及保存2、12、24和48小时后采集后一组器官的样本。对所有样本进行针对细胞间黏附分子-1、血管细胞黏附分子-1和II类主要组织相容性复合体分子的单克隆抗体免疫染色，并评估这些决定簇的相对存在情况。
在对照组和保存的肺中，细胞间黏附分子-1在间隔毛细血管内皮和肺泡上皮细胞中表达强烈，但在支气管上皮中基本不表达。血管细胞黏附分子-1在所有类型的大、小血管内皮中呈中度至强阳性标记，在其他细胞类型中未见表达。II类主要组织相容性复合体抗原在肺上皮细胞中可见不同程度表达，但内皮细胞不表达。在指定的保存时间点，同种异体移植物中细胞间黏附分子-1或血管细胞黏附分子-1免疫染色的免疫组织学密度似乎没有显著差异；蛋白质印迹分析证实了这一结论。人类白细胞DR抗原的染色结果也有类似发现。当比较用Euro-Collins溶液灌注的供体肺与用低钾右旋糖酐葡萄糖溶液灌注的供体肺时，这三种分析物的表达同样没有显著差异；在1℃与10℃保存的器官中也是如此。
这些结果表明，在收获后的即刻期间，肺同种异体移植物中细胞间黏附分子-1、血管细胞黏附分子-1或II类主要组织相容性复合体抗原表达的调节不归因于保存条件的影响。
