Johnson E D, Johnson B K, Silverstein D, Tukei P, Geisbert T W, Sanchez A N, Jahrling P B
United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA.
Arch Virol Suppl. 1996;11:101-14. doi: 10.1007/978-3-7091-7482-1_10.
In 1987, an isolated case of fatal Marburg disease was recognized during routine clinical haemorrhagic fever virus surveillance conducted in Kenya. This report describes the isolation and partial characterization of the new Marburg virus (strain Ravn) isolated from this case. The Ravn isolate was indistinguishable from reference Marburg virus strains by cross-neutralization testing. Virus particles and aggregates of Marburg nucleocapsid matrix in Ravn-infected vero cells, were visualized by immunoelectron microscopic techniques, and also in tissues obtained from the patient and from inoculated monkeys. The cell culture isolate produced a haemorrhagic disease typical of Marburg virus infection when inoculated into rhesus monkeys. Disease was characterized by the sudden appearance of fever and anorexia within 4 to 7 days, and death by day 11. Comparison of nucleotide sequences for portions of the glycoprotein genes of Marburg-Ravn were compared with Marburg reference strains Musoki (MUS) and Popp (POP). Nucleotide identity in this alignment between RAV and MUS is 72.3%, RAV and POP is 71%, and MUS and POP is 91.7%. Amino acid identity between RAV and MUS is 72%, RAV and POP is 67%, and MUS and POP is 93%. These data suggest that Ravn is another subtype of Marburg virus, analogous to the emerging picture of a spectrum of Ebola geographic isolates and subtypes.
1987年，在肯尼亚进行的常规临床出血热病毒监测中，发现了一例马尔堡病致死的孤立病例。本报告描述了从该病例中分离出的新型马尔堡病毒（拉夫恩毒株）及其部分特征。通过交叉中和试验，拉夫恩毒株与马尔堡病毒参考毒株无法区分。利用免疫电子显微镜技术，在感染拉夫恩毒株的非洲绿猴肾细胞中以及从患者和接种病毒的猴子身上获取的组织中，观察到了马尔堡病毒核衣壳基质的病毒颗粒和聚集体。将该细胞培养分离株接种到恒河猴体内时，引发了典型的马尔堡病毒感染所致的出血性疾病。疾病特征为在4至7天内突然出现发热和厌食症状，并在第11天死亡。对马尔堡-拉夫恩毒株糖蛋白基因部分的核苷酸序列与马尔堡参考毒株穆索基（MUS）和波普（POP）进行了比较。拉夫恩毒株（RAV）与穆索基毒株（MUS）在此比对中的核苷酸同一性为72.3%，拉夫恩毒株与波普毒株为71%，穆索基毒株与波普毒株为91.7%。拉夫恩毒株与穆索基毒株的氨基酸同一性为72%，拉夫恩毒株与波普毒株为67%，穆索基毒株与波普毒株为93%。这些数据表明，拉夫恩毒株是马尔堡病毒的另一个亚型，类似于埃博拉病毒多种地理分离株和亚型不断出现的情况。
