Mito K
Kawasaki College of Allied Health Professions, Kurashiki, Japan.
Front Med Biol Eng. 1996;7(2):81-92.
This study was carried out to determine the efficacy of photoimmunotherapy using a photosensitizer, lumin, and near-IR laser light irradiation for cancer therapy. Lumin is a potent cell-activating agent and a photosensitizer characterized by typical absorption peaks at 670 and 770 nm. To clarify that ingestion activity is enhanced by lumin administration with different light irradiation, He-Ne laser light (632.8 nm) and near-IR laser light (780 nm) were used. The results showed that a small dose of lumin (5 ng/ml) with near-IR laser light dramatically enhanced the ingestion activity. A human lung cancer grafted onto nude mice was used in this study. After the tumor hypertrophied, lumin was injected into it six times. Then, it was exposed to near-IR laser light (2 mW, 1 min) every other day for 2 weeks. The whole tumor was strongly cicatrized by collagen fiber from the stroma. It was removed 15 weeks after the treatment. Immunological function was investigated using a two-color flow cytometric assay method based on immunofluorescence labeling with specific monoclonal antibodies of 10(4) cell samples harvested from mouse peripheral blood. At 40 weeks, the ratio of T cells to B cells in untreated mice was 0.23, whereas that in treated mice was 0.58. These results demonstrated that photoimmunotherapy with lumin administration and laser light irradiation is a reliable method of cancer therapy.
本研究旨在确定使用光敏剂鲁米诺及近红外激光照射进行光免疫疗法对癌症治疗的疗效。鲁米诺是一种强效细胞激活剂和光敏剂，其特征在于在670和770nm处有典型的吸收峰。为了阐明不同光照射下给予鲁米诺可增强摄取活性，使用了氦氖激光（632.8nm）和近红外激光（780nm）。结果表明，小剂量的鲁米诺（5ng/ml）与近红外激光显著增强了摄取活性。本研究使用了移植到裸鼠身上的人肺癌。肿瘤长大后，向其中注射鲁米诺6次。然后，每隔一天用近红外激光（2mW，1分钟）照射2周。整个肿瘤被来自基质的胶原纤维强烈瘢痕化。治疗后15周将其切除。使用基于用从小鼠外周血收获的10(4)个细胞样本的特异性单克隆抗体进行免疫荧光标记的双色流式细胞术检测方法研究免疫功能。在40周时，未治疗小鼠中T细胞与B细胞的比例为0.23，而治疗小鼠中该比例为0.58。这些结果表明，给予鲁米诺并进行激光照射的光免疫疗法是一种可靠的癌症治疗方法。
