Bednarz J, Thalmann-Goetsch A, Richard G, Engelmann K
Universitäts-Augenklinik Hamburg, Germany.
Ger J Ophthalmol. 1996 May;5(3):127-31.
In this study we determined the influence of vascular endothelial growth factor (VEGF) on bovine corneal endothelial cell proliferation and wound healing. Proliferation was determined by measurement of DNA replication as well as by counting of the number of cells present after a defined growth period. In a wound-healing model, reproducible cell-free areas were created within monolayers of cultured bovine corneal endothelial cells and the migration of the cells into these areas was analyzed. The DNA replication and cell proliferation of bovine corneal endothelial cells were not influenced by VEGF. In contrast, in the wound-healing model, VEGF supplementation at concentrations of 1 and 10 ng/ml increased the cell density of the wounded area by 20% and 50%, respectively, as compared with the cell density of wounds left untreated by VEGF. Furthermore, no increase in DNA replication was found in cells involved in wound healing. Our results demonstrate that healing of bovine corneal endothelial cell layers after wounding is predominantly performed by cell migration rather than by proliferation. This migration can be stimulated by the addition of exogenous VEGF.
在本研究中，我们确定了血管内皮生长因子（VEGF）对牛角膜内皮细胞增殖和伤口愈合的影响。通过测量DNA复制以及在确定的生长周期后对存在的细胞数量进行计数来确定增殖情况。在伤口愈合模型中，在培养的牛角膜内皮细胞单层内创建可重复的无细胞区域，并分析细胞向这些区域的迁移情况。牛角膜内皮细胞的DNA复制和细胞增殖不受VEGF的影响。相比之下，在伤口愈合模型中，与未用VEGF处理的伤口细胞密度相比，添加浓度为1和10 ng/ml的VEGF分别使伤口区域的细胞密度增加了20%和50%。此外，在参与伤口愈合的细胞中未发现DNA复制增加。我们的结果表明，牛角膜内皮细胞层受伤后的愈合主要通过细胞迁移而非增殖来完成。添加外源性VEGF可刺激这种迁移。
