Joseph S A, Tassorelli C, Prasad A V, Lynd-Balta E
Department of Surgery, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
Peptides. 1996;17(4):655-64. doi: 10.1016/0196-9781(96)00063-0.
The subunit proteins p50 and p65 of the transcription factor NF-kappa B inhibitory protein were immunocytochemically identified and mapped in rat brain. The p65 subunit was localized to the cytoplasm of neurons in the lateral hypothalamus and colocalized with alpha-MSH in neurons identified as the alpha-2 component of the alpha-MSH system. The p50 subunit protein was distributed throughout the neocortex, basal ganglia, thalamic, and hypothalamic nuclei, and certain nuclei of the pons and medulla. The I-kappa B protein, which is necessary for the cytoplasmic sequestration of the NF-kappa B transcription factor complex, was identified specifically in regions of limbic, hypothalamic, and autonomic nuclei.
转录因子NF-κB抑制蛋白的亚基蛋白p50和p65通过免疫细胞化学方法在大鼠脑中进行了鉴定和定位。p65亚基定位于下丘脑外侧神经元的细胞质中，并与α-MSH系统的α-2成分所鉴定的神经元中的α-MSH共定位。p50亚基蛋白分布于整个新皮质、基底神经节、丘脑和下丘脑核，以及脑桥和延髓的某些核。对于NF-κB转录因子复合物的细胞质隔离所必需的I-κB蛋白，在边缘系统、下丘脑和自主神经核区域被特异性鉴定。
