Itano M, Tsuchiya S, Morita S, Fujie H, Ishii N, Yanagisawa T, Ohashi Y, Minegishi M, Sugamura K, Konno T
Department of Pediatric Oncology, Tohoku University, Sendai, Japan.
Tohoku J Exp Med. 1996 Apr;178(4):389-98. doi: 10.1620/tjem.178.389.
The IL-2 receptor (IL-2R) gamma chain is shared among receptors for IL-4, IL-7, IL-9 and IL-15 as well as IL-2. In order to clarify the functional role of these cytokines interacting with the common gamma chain in human early hematopoiesis, we studied expression of the IL-2R gamma chain on purified CD34 positive cells from bone marrow and cord blood. Broad populations of bone marrow mononuclear cells were all found to express the IL-2R gamma chain. CD34 positive cells were purified by CD34 monoclonal antibodies and immunomagnetic beads as representative hematopoietic progenitor cells. It was established that only 38 +/- 10% of CD34 positive bone marrow cells (n = 5) and 35 +/- 12% of CD34 positive cord blood cells (n = 11) expressed the IL-2R gamma chain. CD34(+) IL-2R gamma chain(+) and CD34(+) IL-2R gamma chain(-) cells fractionated by cell sorting were subjected to clonogenic assays that showed granulocyte-macrophage colony-forming cells (CFU-GM) were present evenly in both fractions, whereas erythroid burst-forming cells (BFU-E) were enriched in the CD34(+) IL-2R gamma chain(-) fraction approximately two- to six-fold as compared with CD34(+) IL-2R gamma chain(+) fraction. Such clonogenic features did not differ between the bone marrow and cord blood cases. These results indicate that CD34(+) IL-2R gamma chain(-) cells contain immature cells already committed to the erythroid lineage.
白细胞介素-2受体（IL-2R）γ链存在于白细胞介素-4、白细胞介素-7、白细胞介素-9、白细胞介素-15以及白细胞介素-2的受体中。为了阐明这些与共同γ链相互作用的细胞因子在人类早期造血过程中的功能作用，我们研究了骨髓和脐血中纯化的CD34阳性细胞上IL-2Rγ链的表达。发现骨髓单个核细胞的广泛群体均表达IL-2Rγ链。通过CD34单克隆抗体和免疫磁珠纯化CD34阳性细胞，作为代表性的造血祖细胞。结果确定，只有38±10%的CD34阳性骨髓细胞（n = 5）和35±12%的CD34阳性脐血细胞（n = 11）表达IL-2Rγ链。通过细胞分选分离出的CD34(+) IL-2Rγ链(+)和CD34(+) IL-2Rγ链(-)细胞进行克隆形成试验，结果显示粒细胞-巨噬细胞集落形成细胞（CFU-GM）在两个组分中均匀存在，而红系爆式集落形成细胞（BFU-E）在CD34(+) IL-2Rγ链(-)组分中比CD34(+) IL-2Rγ链(+)组分富集约2至6倍。骨髓和脐血病例之间的这种克隆形成特征没有差异。这些结果表明，CD34(+) IL-2Rγ链(-)细胞包含已定向至红系谱系的未成熟细胞。
