钙离子电流激活速率与α1亚基密度相关。
Ca2+ current activation rate correlates with alpha 1 subunit density.
作者信息
Adams B A, Tanabe T, Beam K G
机构信息
Department of Physiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
出版信息
Biophys J. 1996 Jul;71(1):156-62. doi: 10.1016/S0006-3495(96)79212-3.
Abstract
We report here that L-type Ca2+ channels activate rapidly in myotubes expressing current at high density and slowly in myotubes expressing current at low density. Partial block of the current in individual cells does not slow activation, indicating that Ca2+ influx does not link activation rate to current density. Activation rate is positively correlated with the density of gating charge (Qmax) associated with the L-type Ca2+ channels. The range of values for Qmax, and the relationship between activation rate and Qmax, are similar for myotubes expressing native or recombinant L-type Ca2+ channels, whereas peak Ca2+ current density is approximately 3-fold higher for native channels. Taken together, these results suggest that Ca2+ channel density can govern activation kinetics. Our findings have important important implications for studies of ion channel function because they suggest that biophysical properties can be significantly influenced by channel density, both in heterologous expression systems and in native tissues.
摘要
我们在此报告，L型钙通道在高密度表达电流的肌管中快速激活，而在低密度表达电流的肌管中缓慢激活。单个细胞中电流的部分阻断不会减慢激活速度，这表明钙内流并未将激活速率与电流密度联系起来。激活速率与L型钙通道相关的门控电荷密度（Qmax）呈正相关。对于表达天然或重组L型钙通道的肌管，Qmax的值范围以及激活速率与Qmax之间的关系相似，而天然通道的峰值钙电流密度大约高3倍。综上所述，这些结果表明钙通道密度可以控制激活动力学。我们的发现对离子通道功能研究具有重要意义，因为它们表明在异源表达系统和天然组织中，生物物理特性都可能受到通道密度的显著影响。
相似文献
1
Ca2+ current activation rate correlates with alpha 1 subunit density.钙离子电流激活速率与α1亚基密度相关。
Biophys J. 1996 Jul;71(1):156-62. doi: 10.1016/S0006-3495(96)79212-3.
2
Differential regulation of skeletal muscle L-type Ca2+ current and excitation-contraction coupling by the dihydropyridine receptor beta subunit.二氢吡啶受体β亚基对骨骼肌L型钙电流和兴奋-收缩偶联的差异调节
Biophys J. 1999 Apr;76(4):1744-56. doi: 10.1016/S0006-3495(99)77336-4.
3
Molecular origin of the L-type Ca2+ current of skeletal muscle myotubes selectively deficient in dihydropyridine receptor beta1a subunit.骨骼肌肌管中二氢吡啶受体β1a亚基选择性缺陷时L型钙电流的分子起源
Biophys J. 1998 Jul;75(1):207-17. doi: 10.1016/S0006-3495(98)77507-1.
4
The beta subunit increases Ca2+ currents and gating charge movements of human cardiac L-type Ca2+ channels.β亚基可增加人心脏L型钙通道的钙电流和门控电荷移动。
Biophys J. 1996 Mar;70(3):1285-93. doi: 10.1016/S0006-3495(96)79685-6.
5
Heterologous expression of BI Ca2+ channels in dysgenic skeletal muscle.BI钙离子通道在发育不全骨骼肌中的异源表达。
J Gen Physiol. 1994 Nov;104(5):985-96. doi: 10.1085/jgp.104.5.985.
6
Recovery of Ca2+ current, charge movements, and Ca2+ transients in myotubes deficient in dihydropyridine receptor beta 1 subunit transfected with beta 1 cDNA.用β1 cDNA转染的二氢吡啶受体β1亚基缺陷型肌管中Ca2+电流、电荷移动及Ca2+瞬变的恢复情况。
Biophys J. 1997 Aug;73(2):807-18. doi: 10.1016/S0006-3495(97)78113-X.
7
Ca2+-dependent excitation-contraction coupling triggered by the heterologous cardiac/brain DHPR beta2a-subunit in skeletal myotubes.由骨骼肌管中异源性心脏/脑二氢吡啶受体β2a亚基触发的钙依赖性兴奋-收缩偶联。
Biophys J. 2003 Dec;85(6):3739-57. doi: 10.1016/S0006-3495(03)74790-0.
8
Chicken skeletal muscle ryanodine receptor isoforms: ion channel properties.鸡骨骼肌兰尼碱受体亚型：离子通道特性
Biophys J. 1994 Nov;67(5):1834-50. doi: 10.1016/S0006-3495(94)80665-4.
9
Structural elements in domain IV that influence biophysical and pharmacological properties of human alpha1A-containing high-voltage-activated calcium channels.影响含人α1A的高电压激活钙通道生物物理和药理特性的结构域IV中的结构元件。
Biophys J. 1999 Mar;76(3):1384-400. doi: 10.1016/S0006-3495(99)77300-5.
10
Enhanced dihydropyridine receptor channel activity in the presence of ryanodine receptor.在存在兰尼碱受体的情况下增强的二氢吡啶受体通道活性。
Nature. 1996 Mar 7;380(6569):72-5. doi: 10.1038/380072a0.
引用本文的文献
1
Biophysical reviews top five: voltage-dependent charge movement in nerve and muscle.《生物物理评论》前五名：神经和肌肉中电压依赖性电荷移动
Biophys Rev. 2023 Nov 10;15(6):1903-1907. doi: 10.1007/s12551-023-01165-3. eCollection 2023 Dec.
2
Distinct Components of Retrograde Ca(V)1.1-RyR1 Coupling Revealed by a Lethal Mutation in RyR1.由兰尼碱受体1（RyR1）中的致死性突变揭示的逆向钙通道蛋白1.1（Ca(V)1.1）-兰尼碱受体1（RyR1）偶联的不同组分
Biophys J. 2016 Feb 23;110(4):912-21. doi: 10.1016/j.bpj.2015.12.031.
3
The IQ motif is crucial for Cav1.1 function.IQ基序对Cav1.1的功能至关重要。
J Biomed Biotechnol. 2011;2011:504649. doi: 10.1155/2011/504649. Epub 2011 Nov 13.
4
Properties of Na+ currents conducted by a skeletal muscle L-type Ca2+ channel pore mutant (SkEIIIK).骨骼肌 L 型钙通道孔突变体（SkEIIIK）传导的 Na+电流的特性。
Channels (Austin). 2011 May-Jun;5(3):262-8. doi: 10.4161/chan.5.3.15269. Epub 2011 May 1.
5
The calcium channel alpha2/delta1 subunit is involved in extracellular signalling.钙通道α2/δ1亚基参与细胞外信号传导。
J Physiol. 2008 Feb 1;586(3):727-38. doi: 10.1113/jphysiol.2007.147959. Epub 2007 Dec 6.
6
A component of excitation-contraction coupling triggered in the absence of the T671-L690 and L720-Q765 regions of the II-III loop of the dihydropyridine receptor alpha(1s) pore subunit.在二氢吡啶受体α(1s)孔亚基II-III环的T671-L690和L720-Q765区域缺失的情况下触发的兴奋-收缩偶联的一个组成部分。
Biophys J. 2001 Dec;81(6):3294-307. doi: 10.1016/S0006-3495(01)75963-2.
7
Functional expression of the L-type calcium channel in mice skeletal muscle during prenatal myogenesis.产前肌生成过程中小鼠骨骼肌中L型钙通道的功能表达。
Biophys J. 2000 Mar;78(3):1282-92. doi: 10.1016/S0006-3495(00)76684-7.
8
Gating of the L-type Ca channel in human skeletal myotubes: an activation defect caused by the hypokalemic periodic paralysis mutation R528H.人骨骼肌肌管中L型钙通道的门控：低钾性周期性麻痹突变R528H引起的激活缺陷。
J Neurosci. 1998 Dec 15;18(24):10320-34. doi: 10.1523/JNEUROSCI.18-24-10320.1998.
9
G-Protein-dependent facilitation of neuronal alpha1A, alpha1B, and alpha1E Ca channels.G蛋白依赖性促进神经元α1A、α1B和α1E钙通道。
J Neurosci. 1998 Jul 15;18(14):5240-52. doi: 10.1523/JNEUROSCI.18-14-05240.1998.
10
A mutation affecting dihydropyridine-sensitive current levels and activation kinetics in Drosophila muscle and mammalian heart calcium channels.一种影响果蝇肌肉和哺乳动物心脏钙通道中二氢吡啶敏感性电流水平及激活动力学的突变。
J Neurosci. 1998 Apr 1;18(7):2335-41. doi: 10.1523/JNEUROSCI.18-07-02335.1998.
本文引用的文献
1
Dual function of the voltage-dependent Ca2+ channel alpha 2 delta subunit in current stimulation and subunit interaction.电压依赖性Ca2+通道α2δ亚基在电流刺激和亚基相互作用中的双重功能。
Neuron. 1996 Feb;16(2):431-40. doi: 10.1016/s0896-6273(00)80061-6.
2
Voltage-dependent potentiation of L-type Ca2+ channels due to phosphorylation by cAMP-dependent protein kinase.由环磷酸腺苷（cAMP）依赖性蛋白激酶磷酸化所致的L型钙通道电压依赖性增强。
Nature. 1993 Jul 15;364(6434):240-3. doi: 10.1038/364240a0.
3
Critical roles of the S3 segment and S3-S4 linker of repeat I in activation of L-type calcium channels.重复序列I的S3片段和S3-S4连接区在L型钙通道激活中的关键作用。
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1014-8. doi: 10.1073/pnas.91.3.1014.
4
Potentiation by the beta subunit of the ratio of the ionic current to the charge movement in the cardiac calcium channel.心脏钙通道中离子电流与电荷移动比率受β亚基增强。
Science. 1993 Oct 22;262(5133):575-8. doi: 10.1126/science.8211185.
5
Molecular basis for Ca2+ channel diversity.Ca2+通道多样性的分子基础。
Annu Rev Neurosci. 1994;17:399-418. doi: 10.1146/annurev.ne.17.030194.002151.
6
Human neuronal voltage-dependent calcium channels: studies on subunit structure and role in channel assembly.人类神经元电压依赖性钙通道：亚基结构及其在通道组装中作用的研究
Neuropharmacology. 1993 Nov;32(11):1089-102. doi: 10.1016/0028-3908(93)90004-m.
7
Taking the beta test.正在进行beta测试。
Nature. 1994 Mar 3;368(6466):15-6. doi: 10.1038/368015a0.
8
Gating of IsK expressed in Xenopus oocytes depends on the amount of mRNA injected.非洲爪蟾卵母细胞中表达的IsK通道的门控取决于注射的mRNA量。
J Gen Physiol. 1994 Jul;104(1):87-105. doi: 10.1085/jgp.104.1.87.
9
Level of expression in Xenopus oocytes affects some characteristics of a plant inward-rectifying voltage-gated K+ channel.非洲爪蟾卵母细胞中的表达水平会影响一种植物内向整流电压门控钾离子通道的某些特性。
Pflugers Arch. 1994 Oct;428(3-4):422-4. doi: 10.1007/BF00724528.
10
Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits.在HEK293细胞中瞬时表达的心脏L型钙通道的功能特性。α1和β亚基的作用。
J Gen Physiol. 1995 Feb;105(2):289-305. doi: 10.1085/jgp.105.2.289.
Zotero 插件