Chatterjee B, Hopkins J, Dutchak D, Roy A K
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1833-7. doi: 10.1073/pnas.76.4.1833.
Actinomycin D, an inhibitor of DNA-dependent RNA synthesis, increased the hepatic concentration of alpha 2u globulin, an androgen-inducible protein in the rat. Spayed female rats with a marginally induced state of alpha 2u synthesis showed an approximately 5-fold increase in hepatic alpha 2u globulin within 3-6 hr after treatment with actinomycin D. Initial treatment of these animals with 5 alpha-dihydrotestosterone, followed by actinomycin D, resulted within 2-3 hr in a more than 2-fold increase in hepatic alpha 2u globulin compared to animals treated with the androgen alone. In spite of inhibition of hepatic synthesis of poly(A)-containing RNA to less than 25% of control, superinduction with actinomycin D resulted in a parallel increase in the translatable mRNA for alpha 2u globulin. These results showing increase in both alpha 2u globulin and its translatable mRNA after superinduction with actinomycin D support the concept of post-transcriptional repression of alpha2u synthesis.
放线菌素D是一种依赖DNA的RNA合成抑制剂，它能提高大鼠肝脏中α2u球蛋白的浓度，α2u球蛋白是一种雄激素诱导蛋白。处于α2u合成轻度诱导状态的去卵巢雌性大鼠，在用放线菌素D治疗后3至6小时内，肝脏α2u球蛋白浓度增加了约5倍。先用5α-二氢睾酮对这些动物进行预处理，然后再用放线菌素D，与仅用雄激素处理的动物相比，在2至3小时内肝脏α2u球蛋白增加了2倍多。尽管肝脏中含poly(A)的RNA合成被抑制至对照的不到25%，但放线菌素D的超诱导导致α2u球蛋白可翻译mRNA平行增加。这些结果表明，放线菌素D超诱导后α2u球蛋白及其可翻译mRNA均增加，支持了α2u合成的转录后抑制概念。
