Ghosh S, Schroeter D, Paweletz N
Research Program IV, German Cancer Research Center, Heidelberg, Germany.
Exp Cell Res. 1996 Aug 25;227(1):165-9. doi: 10.1006/excr.1996.0262.
Okadaic acid has been shown to induce premature mitosis in HeLa cells belonging to both S-phase and G2-phase. The response is dependent on concentration and perhaps also on the differential susceptibility of cells to okadaic acid (OA). OA resembles caffeine in its ability to override the DNA-replication check point but differs in being capable of inducing premature mitosis in cells in G2-phase, thereby accelerating mitosis. Chromosomes from mitotic cells induced in G2-phase show a typical banding pattern similar to G-banding. From immunoblot experiments and survey of the relevant literature it has been argued that OA may modulate both a tyrosine kinase and a tyrosine phosphatase activity to override the S-phase checkpoint and accelerate the activation of MPF in cells in G2-phase, respectively.
冈田酸已被证明可诱导处于S期和G2期的HeLa细胞过早进行有丝分裂。这种反应取决于浓度，或许还取决于细胞对冈田酸（OA）的不同敏感性。OA在克服DNA复制检查点的能力方面类似于咖啡因，但不同之处在于它能够诱导G2期细胞过早进行有丝分裂，从而加速有丝分裂。在G2期诱导产生的有丝分裂细胞的染色体显示出与G带相似的典型带型。从免疫印迹实验和相关文献调查来看，有人认为OA可能分别调节酪氨酸激酶和酪氨酸磷酸酶的活性，以克服S期检查点并加速G2期细胞中MPF的激活。
