Mitchell B M, Leung A, Stevens J G
Department of Microbiology and Immunology, University of California, Los Angeles, School of Medicine 90024-1747, USA.
Virology. 1996 Sep 1;223(1):198-207. doi: 10.1006/viro.1996.0468.
Cytomegalovirus (CMV), as do other herpesviruses, establishes a lifelong latent infection in its natural host. While in immunologically intact hosts most CMV infections are subclinical, clinical disease follows severe immunosuppression and immunodeficiency. In these situations CMV may produce serious life-threatening disease, and virus reactivated from the latent state is often responsible. Essential to understanding this virus and its pathogenesis is the need to define particular tissue and cell types harboring viral DNA. We searched for viral DNA and RNA in subpopulations of blood cells from mice latently infected with murine CMV by using differential centrifugation and fluorescent antibody cell sorting followed by polymerase chain reaction analysis. Following intravenous inoculation, the viral DNA was found to be present in the buffy coat at and after 21 days postinfection, and both granulocytes and peripheral blood mononuclear leukocytes (PBML) were reservoirs. Further analysis of the PBML fraction by separation into Mac-1+ and Mac-1- cells revealed that monocytes harbored the DNA while lymphocytes were not sites of persistence. We conclude that in buffy coat of latently infected mice the viral DNA is present only in cells of the myeloid lineage. The relationship of this DNA to the latent infection is discussed.
巨细胞病毒（CMV）与其他疱疹病毒一样，在其天然宿主中建立终身潜伏感染。在免疫功能正常的宿主中，大多数CMV感染是亚临床的，但在严重免疫抑制和免疫缺陷的情况下会出现临床疾病。在这些情况下，CMV可能会引发严重的危及生命的疾病，而从潜伏状态重新激活的病毒通常是病因。理解这种病毒及其发病机制的关键在于确定携带病毒DNA的特定组织和细胞类型。我们通过差速离心和荧光抗体细胞分选，然后进行聚合酶链反应分析，在潜伏感染小鼠巨细胞病毒的小鼠血细胞亚群中寻找病毒DNA和RNA。静脉接种后，在感染后21天及之后的血沉棕黄层中发现了病毒DNA，粒细胞和外周血单个核白细胞（PBML）都是病毒储存库。通过将PBML部分分离为Mac-1+和Mac-1-细胞进行进一步分析发现，单核细胞携带DNA，而淋巴细胞不是病毒持续存在的部位。我们得出结论，在潜伏感染小鼠的血沉棕黄层中，病毒DNA仅存在于髓系谱系的细胞中。本文讨论了这种DNA与潜伏感染的关系。
