Yahata T, Kurabayashi T, Honda A, Tojo Y, Tanaka K, Abo T
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
Cell Immunol. 1996 Aug 1;171(2):269-76. doi: 10.1006/cimm.1996.0203.
Bilateral ovariectomy was performed in female C57BL/6 mice to investigate whether extrathymic T cells in the liver are regulated by physiological doses of estrogen or progesterone. Extrathymic T cells were identified using a two-color immunofluorescence test for CD3 and interleukin-2 receptor beta-chain (IL-2R beta) antigens. The T cells were identified as intermediate CD3+ cells with a high expression of IL-2R beta. It was further demonstrated that the proportion and number of intermediate CD3+ cells decreased in response to ovariectomy, an effect that was countered by estrogen administration. These results suggest that the activation of extrathymic T cells may be regulated by physiological levels of estrogen.
对雌性C57BL/6小鼠进行双侧卵巢切除术，以研究肝脏中的胸腺外T细胞是否受生理剂量的雌激素或孕激素调节。使用针对CD3和白细胞介素-2受体β链（IL-2Rβ）抗原的双色免疫荧光试验来鉴定胸腺外T细胞。这些T细胞被鉴定为高表达IL-2Rβ的中等CD3+细胞。进一步证明，中等CD3+细胞的比例和数量在卵巢切除术后会下降，而雌激素给药可抵消这种影响。这些结果表明，胸腺外T细胞的激活可能受雌激素生理水平的调节。
