Detection of potential cytokine gene(s) in an Alu-PCR library from the human chromosome 4.

Jumping and linking libraries have been constructed in order to have more information of chromosome 4. Two types of jumping libraries (Not I and Xma III jumping) and one linking library were prepared. The jumping clones represent DNA regions between two rare-cutting enzymes sites (such is Not I or Xma III) whereas linking clones represent only the area which surrounds the rare-cutting site. These clones were used as target DNA in Alu-PCR. The Alu-PCR fragments were cloned into pGEM-T vector and transformed into Escherichia coli JM 109 competent cells. The colonies were screened for inserts by restriction analysis. More than 50% of the colonies were shown to contain inserts. Inserts from each library were screened by PCR. One of the jumping libraries appeared to have inserts containing CpG islands and AT-rich sequences.