Baron E J, Weber D E, Weide L G
Department of Medicine, University of California, Los Angeles, USA.
Clin Diagn Lab Immunol. 1996 Jul;3(4):429-31. doi: 10.1128/cdli.3.4.429-431.1996.
Two commercial enzyme-linked immunosorbent assays (ELISAs) for antibodies associated with development of insulin-dependent (type 1) diabetes mellitus (IDDM) were evaluated in conjunction with a conventional indirect immunofluorescent-antibody-islet cell antibody (ICA) test and a radioimmunoprecipitation method for detection of insulin autoantibodies in sera from a selected group of patients. The anti-ICA ELISA was positive for only 1 to 17 serum samples from newly diagnosed IDDM patients but yielded false-positive results with 2 of 6 serum samples containing non-diabetes-related autoantibodies. Although the anti-glutamic acid decarboxylase ELISA did not show positive results for sera with other autoantibodies, it was positive for only 4 of 29 serum samples from recently diagnosed IDDM patients and for 49% of 37 indirect immunofluorescent-antibody-ICA test-positive sera. Until the antibodies associated with the development of diabetes are better characterized, allowing better standards for comparison, it will be difficult to evaluate commercial assays in this field.
对两种用于检测与胰岛素依赖型（1型）糖尿病（IDDM）发病相关抗体的商用酶联免疫吸附测定（ELISA），结合传统的间接免疫荧光抗体胰岛细胞抗体（ICA）检测以及放射免疫沉淀法，对一组特定患者血清中的胰岛素自身抗体进行了评估。抗ICA ELISA仅对新诊断的IDDM患者的1至17份血清样本呈阳性，但对6份含有非糖尿病相关自身抗体的血清样本中的2份产生了假阳性结果。尽管抗谷氨酸脱羧酶ELISA对含有其他自身抗体的血清未显示阳性结果，但对最近诊断的IDDM患者的29份血清样本中仅4份呈阳性，对37份间接免疫荧光抗体-ICA检测呈阳性的血清样本中的49%呈阳性。在与糖尿病发病相关的抗体得到更好的表征，从而有更好的比较标准之前，很难评估该领域的商用检测方法。
