Hudock J A, Hanau C A, Christen R, Bibbo M
Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University Hospital, Philadelphia, PA, USA.
Diagn Cytopathol. 1996 Jul;15(1):78-83. doi: 10.1002/(SICI)1097-0339(199607)15:1<78::AID-DC17>3.0.CO;2-9.
Estrogen and progesterone receptor reactivity may be useful in identifying possible primary sites of metastatic disease or directing therapy in tumors of the female genital tract, including breast, ovary, and endometrium. Various methods have been described for the immunocytochemical evaluation of estrogen receptor (ER) and progesterone receptor (PR) status of cytologic specimens but our results have been variable. We evaluated the effectiveness of various fixatives [cytospin collection fluid, Shandon, Pittsburgh, PA (SH); ethanol (ETH); and formalin (FOR)] for fixation of smears (SM) and cell block (CB) material. The percentage and intensity of tumor nuclei of SM, CB, and tissue sections (TS) stained for ER and PR by the avidin-biotin-peroxidase complex technique were compared. Samples were considered ER or PR positive when > or = 20% of tumor nuclei were stained. The sensitivity of ER analysis of SMs and CBs in each fixative compared to formalin-fixed paraffin-embedded tissue sections were as follows: SM (SH) 88%, SM (ETH) 14%, CB (SH) 58%, CB (ETH) 43%, and CB (FOR) 70%. The sensitivity of PR determination on SMs and CBs was SM (SH) 71%, SM (ETH) 6.0%, CB (SH) 25%, CB (ETH) 33%, CB (FOR) 80%. These findings indicate that of the fixatives evaluated for ER analysis, SMs fixed in SH provided the best results. For PR evaluation, CBs fixed in FOR gave the best results.
雌激素和孕激素受体反应性可能有助于识别转移性疾病的可能原发部位或指导女性生殖道肿瘤（包括乳腺、卵巢和子宫内膜）的治疗。已经描述了多种用于免疫细胞化学评估细胞学标本中雌激素受体（ER）和孕激素受体（PR）状态的方法，但我们的结果一直存在差异。我们评估了各种固定剂[细胞涂片收集液，Shandon，匹兹堡，宾夕法尼亚州（SH）；乙醇（ETH）；和福尔马林（FOR）]对涂片（SM）和细胞块（CB）材料的固定效果。比较了通过抗生物素蛋白-生物素-过氧化物酶复合物技术对SM、CB和组织切片（TS）进行ER和PR染色的肿瘤细胞核的百分比和强度。当≥20%的肿瘤细胞核被染色时，样本被认为ER或PR阳性。与福尔马林固定的石蜡包埋组织切片相比，每种固定剂中SM和CB的ER分析灵敏度如下：SM（SH）88%，SM（ETH）14%，CB（SH）58%，CB（ETH）43%，CB（FOR）70%。SM和CB上PR测定的灵敏度为SM（SH）71%，SM（ETH）6.0%，CB（SH）25%，CB（ETH）33%，CB（FOR）80%。这些发现表明，在评估用于ER分析的固定剂中，用SH固定的SM提供了最佳结果。对于PR评估，用FOR固定的CB给出了最佳结果。
