Vazquez N, Buckley H R, Rogers T J
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA, USA.
J Interferon Cytokine Res. 1996 Jun;16(6):465-70. doi: 10.1089/jir.1996.16.465.
Treatment of the cell wall of Candida albicans with ethylenediamine yields an extract that is antigenic for both the humoral and cell-mediated arms of the immune system. This extract has been shown in previous studies by this laboratory and others to possess potent immunomodulatory activity. We report results here that show that treatment of the macrophage-like cell line RAW 264.7 with the ethylenediamine cell wall (EDA-CW) extract results in an increase in the production of both interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha). Our results also show that the EDA-CW extract possess potent costimulatory activity when combined with interferon-gamma (IFN-gamma). We have found, on the other hand, that EDA-CW extract-treated cells fail to produce elevated levels of IL-1 either alone or in combination with IFN-gamma as a costimulus. Our analysis also shows that the activation of TNF-alpha production by the EDA-CW extract appears to be at the level of transcription, since Northern blot analysis shows that the increase in the level of TNF-alpha mRNA is essentially identical to the rise in TNF-alpha activity released. We suggest that a component of the immunomodulatory activity of the EDA-CW extract is via the activation of macrophage function.
用乙二胺处理白色念珠菌的细胞壁可得到一种提取物，该提取物对免疫系统的体液免疫和细胞介导免疫分支均具有抗原性。本实验室及其他研究机构之前的研究表明，这种提取物具有强大的免疫调节活性。我们在此报告的结果显示，用乙二胺细胞壁（EDA-CW）提取物处理巨噬细胞样细胞系RAW 264.7会导致白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的产生均增加。我们的结果还表明，EDA-CW提取物与干扰素-γ（IFN-γ）联合使用时具有强大的共刺激活性。另一方面，我们发现，单独用EDA-CW提取物处理的细胞或与IFN-γ作为共刺激剂联合处理的细胞均不会产生升高水平的IL-1。我们的分析还表明，EDA-CW提取物对TNF-α产生的激活似乎发生在转录水平，因为Northern印迹分析显示TNF-α mRNA水平的增加与释放的TNF-α活性的升高基本一致。我们认为，EDA-CW提取物免疫调节活性的一个组成部分是通过激活巨噬细胞功能实现的。
