Kumar K, Ballantyne J A, Baker A B
School of Pharmacy, University of Otago, Dunedin, New Zealand.
J Pharm Biomed Anal. 1996 Apr;14(6):667-73. doi: 10.1016/0731-7085(95)01685-6.
A reversed-phase high-performance liquid chromatography method for the simultaneous determination of plasma concentrations of the narcotic analgesics alfentanil and fentanyl using papaverine hydrochloride as the internal standard is presented. Chromatographic separations were achieved with an Econosphere CN, 5 microns, 25 cm x 4.6 mm i.d. column and the effluent was monitored at 195 nm. The assay was linear over the clinically relevant plasma range of 2-2000 ng ml-1 for alfentanil and 2-100 ng ml-1 for fentanyl and has the sensitivity and specificity necessary to determine plasma concentrations of these compounds. Inter- and intra-day precision (RSD) for both compounds did not exceed 10% in these ranges. The assay procedure was utilized for pharmacokinetic studies of plasma concentrations in subjects receiving alfentanil and fentanyl during and after cardiac surgery. This will allow better elucidation of pharmacokinetic variables in this populace.
本文介绍了一种反相高效液相色谱法，以盐酸罂粟碱为内标，同时测定血浆中麻醉性镇痛药阿芬太尼和芬太尼的浓度。采用粒径为5微米、内径为4.6毫米、长25厘米的Econosphere CN柱进行色谱分离，流出物在195纳米处进行监测。该测定法在阿芬太尼2 - 2000纳克/毫升和芬太尼2 - 100纳克/毫升的临床相关血浆范围内呈线性，具有测定这些化合物血浆浓度所需的灵敏度和特异性。在这些范围内，两种化合物的日间和日内精密度（相对标准偏差）均不超过10%。该测定程序用于心脏手术期间及术后接受阿芬太尼和芬太尼的受试者血浆浓度的药代动力学研究。这将有助于更好地阐明该人群的药代动力学变量。
