环孢素A和三氟拉嗪对大鼠腮腺淀粉酶体外分泌的影响。

The effect of cyclosporine A and trifluoperazine on amylase secretion from rat parotid glands in vitro.

作者信息

Samini M, Dehpour A R, Ghafourifar P, Hassanzadeh P

机构信息

Department of Pharmacology, Tehran University of Medical Sciences, Iran.

出版信息

Fundam Clin Pharmacol. 1995;9(6):540-4. doi: 10.1111/j.1472-8206.1995.tb00531.x.

DOI:10.1111/j.1472-8206.1995.tb00531.x

PMID:8808174

Abstract

In the present study the effects of trifluoperazine (TFP) and/or cyclosporine A (CsA) on the amylase secretion of rat parotid gland lobules were investigated. Cyclosporine A, in doses of 10(-6), 3 x 10(-6) and 10(-5) M, caused a dose-dependent reduction in amylase secretion. Trifluoperazine, a well-established calmodulin antagonist, also significantly reduced amylase secretion by the gland, confirming that the secretion is calmodulin dependent. Concurrent administration of CsA and TFP showed a marked positive interaction in reducing the secretion of amylase. Thus, the hypothesis that CsA may have Ca2+/calmodulin antagonistic properties is supported by the present study.

摘要

在本研究中，研究了三氟拉嗪（TFP）和/或环孢素A（CsA）对大鼠腮腺小叶淀粉酶分泌的影响。环孢素A剂量为10^(-6)、3×10^(-6)和10^(-5)M时，可导致淀粉酶分泌呈剂量依赖性减少。三氟拉嗪是一种公认的钙调蛋白拮抗剂，也显著降低了腺体的淀粉酶分泌，证实该分泌依赖于钙调蛋白。同时给予CsA和TFP在降低淀粉酶分泌方面显示出明显的正性相互作用。因此，本研究支持CsA可能具有Ca2+/钙调蛋白拮抗特性这一假说。