Carrier A, Rosier M F, Guillemot F, Goguel A F, Pulcini F, Bernheim A, Auffray C, Devignes M D
Génétique Moléculaire et Biologie du Développement, CNRS-UPR 420, Villejuif, France.
Genomics. 1996 Jan 1;31(1):80-9. doi: 10.1006/geno.1996.0012.
We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the 1p13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Généthon collection and seven genes known to map to the 1p13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genic environment.
我们已对1p13区域的11个重叠酵母人工染色体（YAC）进行了特征分析，其中8个包含人类神经生长因子（NGF）基因（HGMW认可符号为NGFB）。已设计出与YAC末端相对应的序列标签位点（STS），并将其用于一组单染色体体细胞杂种的染色体定位，以检查YAC嵌合现象，同时进行荧光原位杂交分析。对YAC末端STS含量的测定和YAC的限制性图谱分析导致构建了一个3兆碱基的YAC重叠群。来自Généthon文库的四个微卫星标记和七个已知定位于1p13区域的基因已在NGF基因周围的重叠群上进行了排序。来自Genexpress目录的一个新基因转录本已定位在重叠群上。这项工作提供了该1号染色体区域的综合物理、遗传和基因图谱。它构成了确定NGF基因结构并进一步表征其基因环境的基础。
