Norman H A, Mischke C F, Allen B, Vincent J S
Weed Science Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.
J Lipid Res. 1996 Jun;37(6):1372-6.
Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic normal phase HPLC with detection at 208 nm. The mobile phase was composed of heptane-isopropanol-0.001 M KCl 40:52:8 (v/v/v). Yields from spinach leaf lipid extracts were 1.8 mg.10 g-1 fresh wt leaf tissue. Molecular species components of purified sulfoquinovosyldiacylglycerol were separated by reversed-phase C18 HPLC, and fatty acid positional distribution was defined.
本文描述了从植物叶片组织脂质提取物中半制备分离磺基喹喔啉二酰基甘油以及分析其组分分子种类的方法。通过在208nm处检测的等度正相高效液相色谱法，从极性脂质部分的磷脂中分离出磺基喹喔啉二酰基甘油。流动相由庚烷-异丙醇-0.001M氯化钾40:52:8（v/v/v）组成。菠菜叶脂质提取物的产量为1.8mg·10g-1鲜重叶组织。通过反相C18高效液相色谱法分离纯化的磺基喹喔啉二酰基甘油的分子种类组分，并确定脂肪酸的位置分布。
