Ito H O, Shuto T, Takada H, Koga T, Aida Y, Hirata M, Koga T
Department of Oral Biochemistry, Kyushu University School of Dentistry, Fukuoka, Japan.
Arch Oral Biol. 1996 May;41(5):439-44. doi: 10.1016/0003-9969(96)00002-7.
Bacterial lipopolysaccharides possess bone-resorbing activity. Here, lipopolysaccharides from three putative periodontopathic bacteria were examined for effects on osteoclast-like cell formation of bone marrow cells from lipopolysaccharide-responsive C3H-HeN and non-responsive C3H/HeJ mice. The bone marrow cells were cultured with or without various doses of lipopolysaccharide in the presence of 1,25-dihydroxyvitamin D3 and dexamethasone. These lipopolysaccharide preparations significantly increased the number of osteoclast-like cells formed in the culture of C3H/HeN marrow cells; the same as lipopolysaccharides from Escherichia coli and a synthetic lipid A with E. coli-type structure (LA-15-PP), at doses from 0.1 to 1 microgram/ml. This stimulating effect of each lipopolysaccharides was uniformly abrogated by the addition of polymyxin B at 5 micrograms/ml. All the lipopolysaccharide and the synthetic lipid A had no effect on osteoclast formation of the C3H/HeJ marrow cells, whereas lipopolysaccharide from Porphyromonas gingivalis and Prevotella intermedia showed significant mitogenic activity on C3H/HeJ spleen cells. It seems likely that the activity of lipopolysaccharides to augment osteoclast-like cell formation in the bone marrow cell cultures is derived from a common structure of the lipid A portion.
细菌脂多糖具有骨吸收活性。在此，检测了来自三种假定的牙周病原菌的脂多糖对脂多糖反应性C3H-HeN小鼠和无反应性C3H/HeJ小鼠骨髓细胞破骨细胞样细胞形成的影响。在存在1,25-二羟基维生素D3和地塞米松的情况下，将骨髓细胞与不同剂量的脂多糖一起或不与脂多糖一起培养。这些脂多糖制剂显著增加了C3H/HeN骨髓细胞培养物中形成的破骨细胞样细胞的数量；与来自大肠杆菌的脂多糖和具有大肠杆菌型结构的合成脂质A（LA-15-PP）相同，剂量为0.1至1微克/毫升。通过添加5微克/毫升的多粘菌素B，每种脂多糖的这种刺激作用均被消除。所有脂多糖和合成脂质A对C3H/HeJ骨髓细胞的破骨细胞形成均无影响，而牙龈卟啉单胞菌和中间普雷沃菌的脂多糖对C3H/HeJ脾细胞显示出显著的促有丝分裂活性。骨髓细胞培养物中脂多糖增强破骨细胞样细胞形成的活性似乎源自脂质A部分的共同结构。
