Black W D, Hidiroglou M
Department of Biomedical Sciences, University of Guelph, Ontario.
Can J Vet Res. 1996 Jul;60(3):216-21.
Four groups of sheep (5/group) were used in the experiment. Group 1 sheep were given 1 g of ascorbic acid (AA) intravenously (i.v.), group 2 were given 3 g i.v., group 3 were given 1 g intramuscularly (i.m.) and group 4 received 3 g i.m. Blood was collected for 7 h after i.v. administration and for 48 h following i.m. administration. Plasma was analyzed for AA using HPLC techniques. After i.v. administration the rate of elimination was greater at the high dose than the low (0.8560 vs 0.5231 h-1) but the area under the curve (AUC) parameter was proportional to the dosage (127.9 vs 39.7 mcg*h/mL). After i.m. administration AUC parameters were higher than following the i.v. injections. When the times that AA levels were > or = 5 mcg/mL after i.m. injection were compared there was no significant difference between the 1 and 3 g dosages. Times that levels were > or = 10 mcg/mL were significantly longer for the 3 g dose. Using the AUC (area under the curve) parameter as an index of drug exposure, supplementation of adult sheep with AA by the i.m. route should have a greater effect on the animal than i.v. administration.
实验使用了四组绵羊（每组5只）。第1组绵羊静脉注射（i.v.）1克抗坏血酸（AA），第2组静脉注射3克，第3组肌肉注射（i.m.）1克，第4组肌肉注射3克。静脉给药后7小时以及肌肉给药后48小时采集血液。使用高效液相色谱（HPLC）技术分析血浆中的AA。静脉给药后，高剂量组的消除速率高于低剂量组（0.8560对0.5231 h-1），但曲线下面积（AUC）参数与剂量成正比（127.9对39.7 mcg*h/mL）。肌肉给药后的AUC参数高于静脉注射后。比较肌肉注射后AA水平≥5 mcg/mL的时间，1克和3克剂量组之间无显著差异。3克剂量组中AA水平≥10 mcg/mL的时间显著更长。以AUC（曲线下面积）参数作为药物暴露指标，成年绵羊通过肌肉注射途径补充AA对动物的影响应大于静脉给药。
