Li H, Liu S, Kemper B
Department of Cell and Structural Biology, College of Medicine, University of Illinois at Urbana-Champaign 61801, USA.
Mol Cell Endocrinol. 1996 Jun 18;120(1):77-83. doi: 10.1016/0303-7207(96)03823-3.
To study the transcriptional regulation of the rabbit cytochrome P450 2C2 gene (CYP2C2), transgenic mice were generated using a DNA fragment containing the CYP2C2 5'-flanking region fused to a luciferase reporter gene. In one of three transgenic lines, this transgene was expressed in the liver and at a lower level in the kidney, similar to the tissue-specific pattern of this gene in rabbits. Unlike expression in rabbits, the transgene was expressed in a male-specific pattern in liver and kidney, and in the brain of both sexes. Expression of the transgene in male mice was repressed after castration, while expression in females was stimulated after testosterone treatment and periodic injection of rat growth hormone (GH). The expression of this transgene was also regulated developmentally in the liver. These results suggest that this 3500 bp of CYP2C2 5'-flanking region contains the basal transcriptional regulation elements for tissue-specific and developmental expression. Although a position effect can not be ruled out, this transgene may also include the cis-regulatory element for sexual dimorphic expression.
为了研究兔细胞色素P450 2C2基因（CYP2C2）的转录调控，利用一个包含与荧光素酶报告基因融合的CYP2C2 5'-侧翼区的DNA片段制备了转基因小鼠。在三个转基因品系中的一个中，该转基因在肝脏中表达，在肾脏中表达水平较低，类似于该基因在兔中的组织特异性模式。与在兔中的表达不同，该转基因在肝脏和肾脏中以雄性特异性模式表达，并且在两性的大脑中也有表达。阉割后雄性小鼠中转基因的表达受到抑制，而雌性小鼠在睾酮处理和定期注射大鼠生长激素（GH）后转基因的表达受到刺激。该转基因在肝脏中的表达也受到发育调控。这些结果表明，CYP2C2 5'-侧翼区的这3500 bp包含组织特异性和发育表达的基础转录调控元件。虽然不能排除位置效应，但该转基因也可能包括性别二态性表达的顺式调控元件。
