Drén C, Farkas T, Németh I
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.
Vet Microbiol. 1996 May;50(1-2):7-16. doi: 10.1016/0378-1135(96)00002-8.
A serological survey on the prevalence of chicken anaemia virus (CAV) infection was performed by using the indirect immunofluorescence (IF) and the virus neutralization (VN) tests in commercial Hungarian chicken populations. By the indirect IF test, a total of 846 serum samples from 13 meat-type parent flocks of two breeds were investigated between 10 and 62 weeks of age. All flocks were found to be positive for anti-CAV antibodies, and the rates of antibody-positive birds among flocks ranged from 40 to 93.3% and with an average of 73.3%. From nine 1-day- to 9-week-old progeny broiler flocks, 96 serum samples were tested. In the 3 flocks, sampled at the hatchery, 75 to 100% (average 86.4%) of the 22 tested birds were antibody positive. From the 6 flocks, tested between 4 to 9 weeks of age, 3 were antibody negative at 4 and 5 weeks of age, whereas in the 3 remaining 6- to 9-week-old flocks 10 to 20% (average 13.5%) of the birds were antibody positive. In egg-laying parent flocks of 14 and 35 weeks old, 54.4 and 71.7% of the tested 79 and 46 birds had antibodies to CAV, respectively. By the VN test, a total of 670 serum samples from 9 meat-type parent flocks aged between 11 and 37 weeks were investigated. The rate of antibody positivity in flocks was always over 80% and averaged 90.9%. From 7 progeny broiler flocks, 261 serum samples were tested between one day and 7 weeks of age, and the rates of seropositivity in flocks were between 93.3 to 100% and averaged 96.9%. In an egg-laying parent flock, 89.2% of the tested 102 birds had neutralizing antibodies to CAV at 35 weeks old. It was concluded that CAV is widespread in Hungarian commercial chicken populations. Though, according to simultaneous examination of 195 flock sera, the VN test revealed 11.3% more antibody-positive birds, the indirect IF test was found suitable for serological surveys at flock(s) level, provided that sufficient numbers of individual chicks were tested.
利用间接免疫荧光（IF）和病毒中和（VN）试验，对匈牙利商业鸡群中鸡贫血病毒（CAV）感染的流行情况进行了血清学调查。通过间接IF试验，对两个品种的13个肉用型祖代鸡群在10至62周龄期间的846份血清样本进行了检测。所有鸡群均被发现抗CAV抗体呈阳性，各鸡群中抗体阳性鸡的比例在40%至93.3%之间，平均为73.3%。对9个1日龄至9周龄的子代肉鸡群的96份血清样本进行了检测。在孵化场采样的3个鸡群中，22只被检测鸡的抗体阳性率为75%至100%（平均86.4%）。在4至9周龄期间检测的6个鸡群中，有3个鸡群在4周龄和5周龄时抗体呈阴性，而在其余3个6至9周龄的鸡群中，10%至20%（平均13.5%）的鸡抗体呈阳性。在14周龄和35周龄的产蛋祖代鸡群中，79只和46只被检测鸡的抗体阳性率分别为54.4%和71.7%。通过VN试验，对9个11至37周龄的肉用型祖代鸡群的670份血清样本进行了检测。鸡群中的抗体阳性率始终超过80%，平均为90.9%。对7个子代肉鸡群在1日龄至7周龄期间的261份血清样本进行了检测，鸡群中的血清阳性率在93.3%至100%之间，平均为96.9%。在一个35周龄的产蛋祖代鸡群中，102只被检测鸡中有89.2%对CAV具有中和抗体。得出的结论是，CAV在匈牙利商业鸡群中广泛存在。不过，根据对195个鸡群血清的同时检测，VN试验显示抗体阳性鸡多11.3%，但发现间接IF试验适用于鸡群水平的血清学调查，前提是检测足够数量的个体雏鸡。
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