Derbyshire M K, Weinstock K G, Strathern J N
Gene Regulation and Chromosome Biology Laboratory, NCI-Frederick Cancer Research and Development Center, ABL-Basic Research Program, Maryland 21702-1201, USA.
Yeast. 1996 Jun 15;12(7):631-40. doi: 10.1002/(SICI)1097-0061(19960615)12:7%3C631::AID-YEA960%3E3.0.CO;2-8.
A novel Saccharomyces cerevisiae gene, HST1, was identified from among anonymous cDNAs and the complete corresponding genomic clone was isolated and sequenced. HST1 is very closely related to SIR2, showing 71% sequence identity over 84% of its length. Polymerase chain reaction with degenerate primers on S. cerevisiae DNA identified three additional SIR2-related genes designated HST2, HST3 and HST4. The sequences of HST2, HST3 and HST4 correspond to sequences previously released by the S. cerevisiae genome sequencing project as U33335, NCBI gi:965078; X87331, NCBI gi:829135; and Z48784, YD9346.03, respectively. Disruption of HST1 has shown no phenotype with respect to mechanisms in which SIR2 has a role, namely, regional silencing of HML alpha, or in rDNA recombination.
从无名cDNA中鉴定出一个新的酿酒酵母基因HST1，并分离出完整的相应基因组克隆并进行测序。HST1与SIR2密切相关，在其84%的长度上显示出71%的序列同一性。用简并引物对酿酒酵母DNA进行聚合酶链反应，鉴定出另外三个与SIR2相关的基因，分别命名为HST2、HST3和HST4。HST2、HST3和HST4的序列分别对应于酿酒酵母基因组测序项目先前发布的序列U33335（NCBI gi:965078）、X87331（NCBI gi:829135）和Z48784（YD9346.03）。HST1的破坏在SIR2起作用的机制方面没有表现出任何表型，即HMLα的区域沉默或rDNA重组。
