通过体外筛选的2'-氨基修饰RNA对人促甲状腺激素（hTSH）的高亲和力和特异性识别。
High-affinity and specific recognition of human thyroid stimulating hormone (hTSH) by in vitro-selected 2'-amino-modified RNA.
作者信息
Lin Y, Nieuwlandt D, Magallanez A, Feistner B, Jayasena S D
机构信息
NeXstar Pharmaceuticals, Inc., Boulder, CO 80301, USA.
出版信息
Nucleic Acids Res. 1996 Sep 1;24(17):3407-14. doi: 10.1093/nar/24.17.3407.
Abstract
RNA sequences containing 2'-amino pyrimidines that bind with high-affinity to human thyroid stimulating hormone (hTSH) were isolated from a random sequence library by an in vitro selection-amplification procedure. A representative RNA ligand (T-15) has an equilibrium dissociation constant (Kd) of 2.5 nM for its interaction with hTSH and can discriminate between other members of the glycohormone family; no detectable binding was observed at low micromolar concentrations of hCG (human chorionic gonadotropin), while measured Kd values for the interactions with hLH (human leutinizing hormone) and hFSH (human follicle stimulating hormone) were > 1 microM and approximately 0.2 microM, respectively. The detection of hTSH in a dot blot assay with radiolabeled T-15 RNA was demonstrated.
摘要
通过体外筛选-扩增程序，从随机序列文库中分离出与人类促甲状腺激素（hTSH）具有高亲和力结合的含2'-氨基嘧啶的RNA序列。一种代表性的RNA配体（T-15）与hTSH相互作用的平衡解离常数（Kd）为2.5 nM，并且能够区分糖激素家族的其他成员；在低微摩尔浓度的hCG（人绒毛膜促性腺激素）下未观察到可检测的结合，而与hLH（人促黄体生成素）和hFSH（人促卵泡激素）相互作用的测定Kd值分别>1 microM和约0.2 microM。用放射性标记的T-15 RNA进行的斑点印迹分析证明了hTSH的检测。
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