Suppr超能文献

质粒pAMβ1 解离系统的体内分析

In vivo analysis of the plasmid pAM beta 1 resolution system.

作者信息

Jannière L, McGovern S, Pujol C, Petit M A, Ehrlich S D

机构信息

Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

出版信息

Nucleic Acids Res. 1996 Sep 1;24(17):3431-6. doi: 10.1093/nar/24.17.3431.

DOI:10.1093/nar/24.17.3431
PMID:8811099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146103/
Abstract

The promiscuous plasmid pAM beta 1 from Gram-positive bacteria encodes a resolution system which differs from that of Tn3 in that (i) it requires a histone-like protein and an unusual resolvase-DNA interaction to promote recombination and (ii) it mediates in vivo DNA inversion in plasmid substrates. In this in vivo analysis, the pAM beta 1 resolution site is narrowed down to a 99 bp segment, the strand exchange is mapped within 10 bp and the serine residue at position 10 of the resolvase is shown to be essential for enzyme activity. In addition, data showing that the resolution system does not promote DNA inversion in the Bacillus subtilis chromosome are presented. Implications of this observation are discussed.

摘要

革兰氏阳性菌中的多拷贝质粒pAMβ1编码一种解离系统，该系统与Tn3的解离系统不同，具体表现为：（i）它需要一种类组蛋白和一种特殊的解离酶 - DNA相互作用来促进重组；（ii）它在质粒底物中介导体内DNA倒位。在这项体内分析中，pAMβ1解离位点被缩小到一个99 bp的片段，链交换定位在10 bp范围内，并且解离酶第10位的丝氨酸残基被证明对酶活性至关重要。此外，还展示了该解离系统不会促进枯草芽孢杆菌染色体中DNA倒位的数据。讨论了这一观察结果的意义。

相似文献

1
In vivo analysis of the plasmid pAM beta 1 resolution system.质粒pAMβ1 解离系统的体内分析
Nucleic Acids Res. 1996 Sep 1;24(17):3431-6. doi: 10.1093/nar/24.17.3431.
2
The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the beta recombinase of plasmid pSM19035.枯草芽孢杆菌类组蛋白Hbsu是质粒pSM19035的β重组酶介导的DNA拆分和DNA倒位所必需的。
J Biol Chem. 1995 Feb 17;270(7):2938-45. doi: 10.1074/jbc.270.7.2938.
3
A novel site-specific recombinase encoded by the Streptococcus pyogenes plasmid pSM19035.化脓性链球菌质粒pSM19035编码的一种新型位点特异性重组酶。
J Mol Biol. 1994 Apr 29;238(2):159-72. doi: 10.1006/jmbi.1994.1278.
4
The beta recombinase of plasmid pSM19035 binds to two adjacent sites, making different contacts at each of them.质粒pSM19035的β重组酶与两个相邻位点结合，在每个位点形成不同的接触。
Nucleic Acids Res. 1995 Aug 25;23(16):3181-8. doi: 10.1093/nar/23.16.3181.
5
pAM beta 1 resolvase has an atypical recombination site and requires a histone-like protein HU.pAM beta 1 解离酶具有一个非典型的重组位点，并且需要一种类组蛋白HU。
Mol Microbiol. 1995 Oct;18(2):271-82. doi: 10.1111/j.1365-2958.1995.mmi_18020271.x.
6
[Determination of the minimal length DNA homologous region required for plasmid integration into the Bacillus subtilis chromosome via homologous recombination].[通过同源重组将质粒整合到枯草芽孢杆菌染色体所需的最小长度DNA同源区域的测定]
Genetika. 1992 Jul;28(7):38-45.
7
Identification and characterization of the dif Site from Bacillus subtilis.枯草芽孢杆菌中dif位点的鉴定与表征
J Bacteriol. 2001 Feb;183(3):1058-68. doi: 10.1128/JB.183.3.1058-1068.2001.
8
Characterization of a region of the Enterococcus faecalis plasmid pAM beta 1 which enhances the segregational stability of pAM beta 1-derived cloning vectors in Bacillus subtilis.粪肠球菌质粒pAMβ1中一个区域的特性，该区域可增强pAMβ1衍生的克隆载体在枯草芽孢杆菌中的分离稳定性。
Plasmid. 1991 Nov;26(3):209-21. doi: 10.1016/0147-619x(91)90044-w.
9
Molecular characterization of the resolvase gene, res, carried by a multicopy plasmid from Clostridium perfringens: common evolutionary origin for prokaryotic site-specific recombinases.由产气荚膜梭菌多拷贝质粒携带的解离酶基因res的分子特征：原核生物位点特异性重组酶的共同进化起源
Mol Microbiol. 1987 Nov;1(3):371-6. doi: 10.1111/j.1365-2958.1987.tb01944.x.
10
Mechanism of Tn3 resolvase recombination in vivo.
J Biol Chem. 1991 Feb 5;266(4):2041-7.

引用本文的文献

1
Novel shuttle vector pGMβ1 for conjugative chromosomal manipulation of subsp. .用于亚种接合染色体操作的新型穿梭载体pGMβ1 。 （你提供的原文似乎不完整，“subsp.”后面缺少具体内容）
Biosci Microbiota Food Health. 2022;41(1):20-29. doi: 10.12938/bmfh.2021-014. Epub 2021 Oct 18.
2
Site-specific DNA Inversion by Serine Recombinases.丝氨酸重组酶介导的位点特异性DNA倒位
Microbiol Spectr. 2015 Feb 19;3(3):1-36. doi: 10.1128/microbiolspec.MDNA3-0047-2014.

本文引用的文献

1
The role of chromatin-associated protein Hbsu in beta-mediated DNA recombination is to facilitate the joining of distant recombination sites.染色质相关蛋白Hbsu在β介导的DNA重组中的作用是促进远距离重组位点的连接。
Mol Microbiol. 1995 Nov;18(3):471-8. doi: 10.1111/j.1365-2958.1995.mmi_18030471.x.
2
pAM beta 1 resolvase has an atypical recombination site and requires a histone-like protein HU.pAM beta 1 解离酶具有一个非典型的重组位点，并且需要一种类组蛋白HU。
Mol Microbiol. 1995 Oct;18(2):271-82. doi: 10.1111/j.1365-2958.1995.mmi_18020271.x.
3
The Hin dimer interface is critical for Fis-mediated activation of the catalytic steps of site-specific DNA inversion.Hin二聚体界面对于Fis介导的位点特异性DNA倒位催化步骤的激活至关重要。
Curr Biol. 1996 Feb 1;6(2):163-77. doi: 10.1016/s0960-9822(02)00449-9.
4
Contributions of supercoiling to Tn3 resolvase and phage Mu Gin site-specific recombination.超螺旋对Tn3解离酶和噬菌体Mu Gin位点特异性重组的作用。
J Mol Biol. 1996 Feb 16;256(1):50-65. doi: 10.1006/jmbi.1996.0067.
5
Analysis of the stabilization system of pSM19035-derived plasmid pBT233 in Bacillus subtilis.枯草芽孢杆菌中源自pSM19035的质粒pBT233稳定系统的分析
Gene. 1993 Dec 22;136(1-2):1-12. doi: 10.1016/0378-1119(93)90441-5.
6
recA-independent and recA-dependent intramolecular plasmid recombination. Differential homology requirement and distance effect.不依赖RecA和依赖RecA的分子内质粒重组。差异同源性要求和距离效应。
J Mol Biol. 1994 Jan 14;235(2):414-23. doi: 10.1006/jmbi.1994.1002.
7
A fourth class of theta-replicating plasmids: the pAM beta 1 family from gram-positive bacteria.第四类θ复制质粒：来自革兰氏阳性菌的pAMβ1家族。
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11668-72. doi: 10.1073/pnas.90.24.11668.
8
The promiscuous plasmids pIP501 and pAM beta 1 from gram-positive bacteria encode complementary resolution functions.来自革兰氏阳性菌的杂乱质粒pIP501和pAMβ1编码互补的解离功能。
Plasmid. 1994 Jan;31(1):100-5. doi: 10.1006/plas.1994.1010.
9
A novel site-specific recombinase encoded by the Streptococcus pyogenes plasmid pSM19035.化脓性链球菌质粒pSM19035编码的一种新型位点特异性重组酶。
J Mol Biol. 1994 Apr 29;238(2):159-72. doi: 10.1006/jmbi.1994.1278.
10
Gene organization of the Streptococcus pyogenes plasmid pDB101: sequence analysis of the orf eta-copS region.化脓性链球菌质粒pDB101的基因组织：orf eta-copS区域的序列分析
Gene. 1994 Jul 22;145(1):33-9. doi: 10.1016/0378-1119(94)90319-0.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验