质粒pAMβ1 解离系统的体内分析

In vivo analysis of the plasmid pAM beta 1 resolution system.

作者信息

Jannière L, McGovern S, Pujol C, Petit M A, Ehrlich S D

机构信息

Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

出版信息

Nucleic Acids Res. 1996 Sep 1;24(17):3431-6. doi: 10.1093/nar/24.17.3431.

DOI:10.1093/nar/24.17.3431

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146103/

Abstract

The promiscuous plasmid pAM beta 1 from Gram-positive bacteria encodes a resolution system which differs from that of Tn3 in that (i) it requires a histone-like protein and an unusual resolvase-DNA interaction to promote recombination and (ii) it mediates in vivo DNA inversion in plasmid substrates. In this in vivo analysis, the pAM beta 1 resolution site is narrowed down to a 99 bp segment, the strand exchange is mapped within 10 bp and the serine residue at position 10 of the resolvase is shown to be essential for enzyme activity. In addition, data showing that the resolution system does not promote DNA inversion in the Bacillus subtilis chromosome are presented. Implications of this observation are discussed.

摘要

革兰氏阳性菌中的多拷贝质粒pAMβ1编码一种解离系统，该系统与Tn3的解离系统不同，具体表现为：（i）它需要一种类组蛋白和一种特殊的解离酶 - DNA相互作用来促进重组；（ii）它在质粒底物中介导体内DNA倒位。在这项体内分析中，pAMβ1解离位点被缩小到一个99 bp的片段，链交换定位在10 bp范围内，并且解离酶第10位的丝氨酸残基被证明对酶活性至关重要。此外，还展示了该解离系统不会促进枯草芽孢杆菌染色体中DNA倒位的数据。讨论了这一观察结果的意义。

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本文引用的文献

1

The role of chromatin-associated protein Hbsu in beta-mediated DNA recombination is to facilitate the joining of distant recombination sites.染色质相关蛋白Hbsu在β介导的DNA重组中的作用是促进远距离重组位点的连接。

Mol Microbiol. 1995 Nov;18(3):471-8. doi: 10.1111/j.1365-2958.1995.mmi_18030471.x.

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pAM beta 1 resolvase has an atypical recombination site and requires a histone-like protein HU.pAM beta 1 解离酶具有一个非典型的重组位点，并且需要一种类组蛋白HU。

Mol Microbiol. 1995 Oct;18(2):271-82. doi: 10.1111/j.1365-2958.1995.mmi_18020271.x.

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The Hin dimer interface is critical for Fis-mediated activation of the catalytic steps of site-specific DNA inversion.Hin二聚体界面对于Fis介导的位点特异性DNA倒位催化步骤的激活至关重要。

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recA-independent and recA-dependent intramolecular plasmid recombination. Differential homology requirement and distance effect.不依赖RecA和依赖RecA的分子内质粒重组。差异同源性要求和距离效应。

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A fourth class of theta-replicating plasmids: the pAM beta 1 family from gram-positive bacteria.第四类θ复制质粒：来自革兰氏阳性菌的pAMβ1家族。

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The promiscuous plasmids pIP501 and pAM beta 1 from gram-positive bacteria encode complementary resolution functions.来自革兰氏阳性菌的杂乱质粒pIP501和pAMβ1编码互补的解离功能。

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