Kleemann R, Deschl U, Dietmann G, Wilhelms O H, Juchem R, Rebel W, Hartig F
Diabetes-Forschungsinstitut an der Heinrich-Heine Universität Düsseldorf, Klinische Abteilung, Germany.
Exp Toxicol Pathol. 1996 Jun;48(4):233-41. doi: 10.1016/S0940-2993(96)80004-2.
24 hours after an i.v. injection of 2 mg Sephadex G 200 particles ovalbumin sensitized Sprague Dawley rats show an antigen specific bronchial hyperreactivity and an unspecific hyperreactivity against serotonin. The aim of this study was to investigate the effects of Sephadex on blood parameters and lung pathology to find the morphological substrate of bronchial hyperreactivity in this animal model. In the blood neutrophilia (p < 0.01) but no eosinophilia was present. We conclude that a blood eosinophilia needs not to be necessarily correlated with hyperreactivity of the airways like claimed by other investigators for this animal model. Histologically we found that Sephadex particles are trapped in smaller-diameter arteries of the lung and lead to a granulomatous arteritis consisting mainly of ED1 positive and widely ED2 negative macrophages interspersed with eosinophils and neutrophils. Larger vessels not occluded by particles showed perivascular oedema with infiltration of eosinophils. We report here for the first time a significant hypertrophy of PAS positive goblet cells (p < 0.01) accompanied by a peribronchial infiltration with eosinophils (p < 0.01) and macrophages positive for ED1, ED2 and Ox-6 (p < 0.01) but not Ox-19 positive T-lymphocytes. The authors suggest that the peribronchial inflammation contributes importantly to the onset of bronchial hyperreactivity in this animal model and that the hypertrophy of goblet cells indicates the pathophysiological importance of peribronchial leukocytes.
给卵清蛋白致敏的Sprague Dawley大鼠静脉注射2毫克葡聚糖G 200颗粒24小时后，出现抗原特异性支气管高反应性以及对血清素的非特异性高反应性。本研究的目的是调查葡聚糖对血液参数和肺部病理的影响，以找到该动物模型中支气管高反应性的形态学基础。血液中存在嗜中性粒细胞增多（p < 0.01），但无嗜酸性粒细胞增多。我们得出结论，血液嗜酸性粒细胞增多不一定与气道高反应性相关，其他研究人员针对该动物模型曾有此说法。组织学上，我们发现葡聚糖颗粒被困在肺的小直径动脉中，并导致肉芽肿性动脉炎，主要由ED1阳性且广泛的ED2阴性巨噬细胞组成，其间散布着嗜酸性粒细胞和嗜中性粒细胞。未被颗粒阻塞的较大血管显示血管周围水肿，伴有嗜酸性粒细胞浸润。我们首次报告了PAS阳性杯状细胞显著肥大（p < 0.01），同时伴有嗜酸性粒细胞（p < 0.01）和ED1、ED2及Ox - 6阳性（p < 0.01）但Ox - 19阳性T淋巴细胞阴性的巨噬细胞的支气管周围浸润。作者认为支气管周围炎症在该动物模型中对支气管高反应性的发生起重要作用，杯状细胞肥大表明支气管周围白细胞的病理生理重要性。
