Kasper M, Schöbl R, Haroske G, Fischer R, Neubert F, Dimmer V, Müller M
Department of Pathology, Technical University Dresden, Germany.
Exp Toxicol Pathol. 1996 Jun;48(4):283-8. doi: 10.1016/S0940-2993(96)80020-0.
To determine the value of von Willebrand factor (vWF) antigen as a marker of endothelial injury in radiation-induced fibrosis of rat lungs, we studied endothelial immunoreactivity to antibodies against vWF using the indirect immunoperoxidase technique combined with morphometric analysis. Using immunoelectron microscopy of LR White embedded lung samples to detect vWF, immunogold-labelled Weibel Palade bodies were found in endothelial cells of capillary endothelium. The irradiated lungs showed a statistically significant elevation of vWF expression, ie, vWF positive endothelia per unit area, and a significant increase of vWF expression per unit of parenchyma as well. The results suggest that vWF antigen expression and the number of vWF positive structures is modulated under condition of injury in radiation-induced fibrosis.
为确定血管性血友病因子（vWF）抗原作为大鼠肺辐射诱导纤维化中内皮损伤标志物的价值，我们采用间接免疫过氧化物酶技术结合形态计量分析，研究了内皮细胞对vWF抗体的免疫反应性。使用LR White包埋的肺样本进行免疫电子显微镜检测vWF，在毛细血管内皮细胞中发现了免疫金标记的魏尔-帕拉德小体。照射后的肺组织中，vWF表达有统计学意义的升高，即单位面积内vWF阳性内皮细胞数量增加，且单位实质组织中vWF表达也显著增加。结果表明，在辐射诱导纤维化的损伤条件下，vWF抗原表达及vWF阳性结构数量受到调节。
