Bertuzzi S, Sheng H Z, Copeland N G, Gilbert D J, Jenkins N A, Taira M, Dawid I B, Westphal H
Laboratory of Mammalian Genes and Development, National Institutes of Health, Bethesda, Maryland, 20892, USA.
Genomics. 1996 Sep 1;36(2):234-9. doi: 10.1006/geno.1996.0458.
Lhx5, the mouse ortholog of the Xenopus Xlim-5, is a LIM/homeobox gene expressed in the central nervous system during both embryonic development and adulthood. During development its domain of expression is mainly localized at the most anterior portion of the neural tube, and it precedes the morphological differentiation of the forebrain; for this reason we believe that Lhx5 could play an important role in forebrain patterning. Here we present the structural organization and the chromosomal localization of the Lhx5 gene. The gene is composed of five exons spanning more than 10 kb of genomic sequence. The first and second LIM domains are encoded by the first and second exon, while the codons of the homeobox are split between the third and the fourth exons. The structure of Lhx5 is similar to that of other LIM/homeodomain proteins, Lhx1/lim1 and Lhx3/lim3, but differs from that of other LIM genes, such as mec3 and LMO1/Rbtn1, in which the codons for the LIM domains are interrupted by introns. We have mapped Lhx5 to the central region of mouse chromosome 5.
Lhx5是非洲爪蟾Xlim-5在小鼠中的同源基因，是一种LIM/同源异型框基因，在胚胎发育和成年期的中枢神经系统中均有表达。在发育过程中，其表达区域主要定位于神经管的最前端，且先于前脑的形态分化；因此我们认为Lhx5可能在前脑模式形成中发挥重要作用。在此我们展示Lhx5基因的结构组织和染色体定位。该基因由五个外显子组成，跨越超过10 kb的基因组序列。第一个和第二个LIM结构域由第一和第二个外显子编码，而同源异型框的密码子则在第三和第四个外显子之间分开。Lhx5的结构与其他LIM/同源结构域蛋白Lhx1/lim1和Lhx3/lim3相似，但与其他LIM基因如mec3和LMO1/Rbtn1不同，在mec3和LMO1/Rbtn1中，LIM结构域的密码子被内含子打断。我们已将Lhx5定位到小鼠5号染色体的中部区域。
