Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat G, Gellin J
Laboratoire de Génétique Cellulaire, Centre de Recherche INRA de Toulouse-Auzeville, Castanet Tolosan CEDEX, 31326, France.
Genomics. 1996 Sep 1;36(2):252-62. doi: 10.1006/geno.1996.0460.
The aim of this study was to determine the correspondence between human and porcine chromosome fragments using whole chromosome painting probes from both species in heterologous hybridization experiments (bidirectional heterologous chromosome painting). Bidirectional experiments allow the determination of segment-to-segment homologies between the chromosomes of these two species. Chromosome-specific painting probes from both species were, except one, obtained by DOP-PCR or PARM-PCR amplification of flow-sorted chromosomes. The probes labeled 95% of the total length of the porcine chromosomes with human painting probes and 60% of the human chromosomes in the reverse experiments. Syntenic relationships of chromosomal segments on the karyotype of both species were determined. There was close agreement between com- parative gene mapping data and the identified homologous segments; this comparison enabled orientation of the segments. We demonstrate that bidirectional heterologous chromosome painting is a highly efficient way of generating comparative cytogenetic maps.
本研究的目的是在异源杂交实验（双向异源染色体涂染）中，使用来自这两个物种的全染色体涂染探针来确定人和猪染色体片段之间的对应关系。双向实验能够确定这两个物种染色体之间片段与片段的同源性。除了一个探针外，来自这两个物种的染色体特异性涂染探针都是通过对流式分选的染色体进行DOP-PCR或PARM-PCR扩增获得的。在反向实验中，用人类涂染探针标记了猪染色体总长度的95%，用猪涂染探针标记了人类染色体的60%。确定了两个物种核型上染色体片段的同线性关系。比较基因定位数据与鉴定出的同源片段之间存在密切一致性；这种比较使片段能够定向排列。我们证明，双向异源染色体涂染是生成比较细胞遗传图谱的一种高效方法。
