Natelson B H, Zhou X, Ottenweller J E, Bergen M T, Sisto S A, Drastal S, Tapp W N, Gause W L
Chronic Fatigue Syndrome Center, UMDNJ-New Jersey Medical School, Newark 07013, USA.
Int J Sports Med. 1996 May;17(4):299-302. doi: 10.1055/s-2007-972850.
Because of recent interest in the effects of physical exercise on immunologic function, we decided to use state-of-the-art methods to evaluate cytokines in the peripheral blood leukocytes (PBLs) of 7 men before and after a maximal treadmill stress test. Change in cytokine gene expression was quantified from PBLs using a reverse transcriptase polymerase chain reaction assay (RT-PCR). In contrast to reports on serum levels or using in vitro testing, direct gene expression of TNF-alpha decreased after the stress test (p < 0.008). However, the 47% decrease was relatively small and of questionable biological significance. Levels of IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, and INF-gamma did not change.
由于近期人们对体育锻炼对免疫功能的影响感兴趣，我们决定使用最先进的方法，对7名男性在进行最大运动强度的跑步机压力测试前后外周血白细胞（PBL）中的细胞因子进行评估。使用逆转录聚合酶链反应分析（RT-PCR）对PBL中的细胞因子基因表达变化进行定量分析。与关于血清水平的报告或体外测试结果相反，压力测试后TNF-α的直接基因表达下降（p < 0.008）。然而，47%的下降幅度相对较小，其生物学意义存疑。IL-1α、IL-1β、IL-2、IL-4、IL-6、IL-10和INF-γ的水平没有变化。
