Ingles P J, Adams P M, Knipple D C, Soderlund D M
Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA.
Insect Biochem Mol Biol. 1996 Apr;26(4):319-26. doi: 10.1016/0965-1748(95)00093-3.
The kdr insecticide resistance trait of the house fly (Musca domestica .L.), which confers reduced neuronal sensitivity to DDT and pyrethroid insecticides, was previously shown to exhibit tight genetic linkage to restriction fragment length polymorphism markers lying within a voltage-sensitive sodium channel gene that is homologous to the para gene of Drosophila melanogaster. In the present study, the 6315 nucleotide coding sequences of this voltage-sensitive sodium channel gene from insecticide-susceptible (NAIDM strain) and kdr (538ge strain) house flies were determined by automated direct DNA sequencing of PCR fragments obtained by amplification on first strand cDNA from adult heads. The deduced 2105-residue amino acid sequence from each strain exhibited overall structure and organization typical of sodium channel alpha subunit genes and was 90.0% identical to that of the D. melanogaster para gene product. We did not detect any splice variants among voltage-sensitive sodium channel cDNAs obtained from adult house fly head preparations. Comparison of the coding sequence of the voltage-sensitive sodium channel gene of the kdr house fly strain to that of the NAIDM strain revealed 12 amino acid differences in the 538ge strain. The significance of these polymorphisms as candidate resistance-conferring mutations is discussed.
家蝇（Musca domestica. L.）的kdr抗杀虫剂特性使其神经元对滴滴涕和拟除虫菊酯类杀虫剂的敏感性降低，先前研究表明，该特性与位于一个电压敏感性钠通道基因内的限制性片段长度多态性标记紧密连锁，该基因与黑腹果蝇的para基因同源。在本研究中，通过对从成年家蝇头部的第一链cDNA扩增得到的PCR片段进行自动直接DNA测序，确定了来自杀虫剂敏感型（NAIDM品系）和kdr（538ge品系）家蝇的该电压敏感性钠通道基因的6315个核苷酸编码序列。每个品系推导的2105个氨基酸残基序列呈现出钠通道α亚基基因典型的整体结构和组织，并且与黑腹果蝇para基因产物的序列有90.0%的同一性。我们在从成年家蝇头部制备物中获得的电压敏感性钠通道cDNA中未检测到任何剪接变体。将kdr家蝇品系的电压敏感性钠通道基因编码序列与NAIDM品系的进行比较，发现在538ge品系中有12个氨基酸差异。讨论了这些多态性作为候选抗性赋予突变的意义。
