Sex differences in the effect of amphetamine on immediate early gene expression in the rat dorsal striatum.

Amphetamine (AMPH)-induced dopamine release in the striatum and AMPH-induced behavior in the rat have been demonstrated to be influenced by sex and hormonal status. The experiments reported here were conducted, therefore, to examine sex differences, hormonal influences and estrous cycle-dependent changes in AMPH-induced immediate early gene expression in the dorsal striatum. Cell counts were taken at three rostrocaudal levels from three to four regions of the dorsal striatum at each level (ventromedial, dorsomedial, dorsolateral, ventrolateral). The immunohistochemical localization of calbindin was used as a control. We report here that females on the afternoon of proestrus had a significantly greater percent of Fos-positive neurons after AMPH across the dorsolateral region of the middle and caudal striatum and in the ventrolateral region of the caudal striatum compared to females in diestrus, ovariectomized (OVX) females, castrated (CAST) males and intact males. There was no difference in AMPH-induced immediate early gene expression between OVX and diestrous rats. There were also no significant differences between CAST and intact males in AMPH-induced Fos expression, with the exception of the ventrolateral caudal striatum. In sum, the present findings indicate that AMPH-induced Fos expression is sexually dimorphic and modulated by gonadal hormones in lateral regions of the rat dorsal striatum.