Haas S, Miura-Fraboni J, Zavala F, Murata K, Leone-Bay A, Santiago N
Emisphere Technologies, Inc., Hawthorne NY, USA.
Vaccine. 1996 Jun;14(8):785-91. doi: 10.1016/0264-410x(95)00243-t.
A model antigen, ovalbumin (OVA), was encapsulated in microspheres prepared from derivatized alpha-amino acids and administered orally to mice. These microspheres are quickly and easily prepared, without the use of organic solvents, high temperatures, or complex purification techniques. Immunological responses included induction of OVA-specific antibodies in both sera (IgG) and in intestinal secretions (sIgA), as well as antigen-dependent proliferation of splenic CD4+ T cells following, in some cases, as little as a single oral priming dose containing 0.1 mg OVA. Oral administration of microspheres was also found to be effective as a secondary immunization following a subcutaneous prime with soluble antigen. In addition, the protective effect of co-encapsulation of cholera toxin, a mucosal adjuvant, was demonstrated in a whole virus model (infectious bursal disease in chickens). These results indicate that oral administration of antigen-loaded derivatized alpha-amino acid microspheres can induce local and systemic antibody production and/or stimulation of effector cells.
将模型抗原卵清蛋白（OVA）包裹于由衍生化α-氨基酸制备的微球中，并口服给予小鼠。这些微球制备迅速且简便，无需使用有机溶剂、高温或复杂的纯化技术。免疫反应包括在血清（IgG）和肠道分泌物（sIgA）中诱导产生OVA特异性抗体，以及在某些情况下，仅单次口服含0.1 mg OVA的初免剂量后，脾CD4+ T细胞发生抗原依赖性增殖。还发现口服微球作为可溶性抗原皮下初免后的二次免疫有效。此外，在全病毒模型（鸡传染性法氏囊病）中证明了共包裹黏膜佐剂霍乱毒素的保护作用。这些结果表明，口服负载抗原的衍生化α-氨基酸微球可诱导局部和全身抗体产生和/或刺激效应细胞。
