Finette G M, Mao Q M, Hearn M T
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
J Chromatogr A. 1996 Aug 30;743(1):57-73. doi: 10.1016/0021-9673(96)00321-4.
In these investigations, a detailed examination of the fluid dynamic characteristics of expanded beds containing silica-based chromatographic adsorbents has been carried out. In particular, the effects of the column accessories such as distributor design and the flow-rate on the dispersion coefficient of the adsorbent particles have been examined. The experimental data have been analysed in terms of residency time effects, fluid flow characteristics and physical properties of the adsorbent particles using several different theoretical models. In common with experience of packed-bed systems, the results confirm that the optimisation of the dynamic capacities as well as the dynamic adsorption rates of adsorbents in expanded-bed systems must take into account column design characteristics as well as the physical/chemical features of the adsorbents, if the highest productivities of expanded-bed/fluidisation procedures are to be achieved with crude feedstocks from biotechnological applications.
在这些研究中，已对含有硅基色谱吸附剂的膨胀床的流体动力学特性进行了详细研究。特别是，已考察了诸如分布器设计和流速等柱配件对吸附剂颗粒分散系数的影响。使用几种不同的理论模型，根据停留时间效应、流体流动特性和吸附剂颗粒的物理性质对实验数据进行了分析。与填充床系统的经验一样，结果证实，如果要利用生物技术应用中的粗原料实现膨胀床/流化过程的最高生产率，那么在膨胀床系统中优化吸附剂的动态容量以及动态吸附速率时，必须考虑柱设计特征以及吸附剂的物理/化学特性。
