A simple and economical procedure for purification of muscle lactate dehydrogenase by batch dye-ligand adsorption.

A simple, economical and rapid purification procedure for muscle lactate dehydrogenase is described. It makes use of batch adsorption on Procion Red H-3B coupled to agarose through an amine linkage. The enzyme is eluted with NAD+ and sulphite, and for many purposes no more processing is necessary. Only 70 ml settled volume of adsorbent is required to process extract containing 1 g of lactate dehydrogenase, which is recovered with up to 90% yield, at over 98% purity. The method is readily scalable up or down, and makes an excellent class demonstration of affinity techniques.