用于回收细胞色素b5的大肠杆菌TB1细胞的冻融和超声处理。
Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery.
作者信息
Santos J A, Belo I, Mota M, Cabral J M
机构信息
Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisboa, Portugal.
出版信息
Bioseparation. 1996 Apr;6(2):81-9.
The influence of sonication power, suspension volume and cell concentration on the kinectics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration.
研究了超声功率、悬浮液体积和细胞浓度对大肠杆菌TB1细胞超声处理后细胞色素b5动力学及细胞内蛋白质释放的影响。还评估了细胞悬浮液冻融的影响。冻融提高了细胞色素b5的回收率。超声处理效率随超声功率的增加、悬浮液体积的减小和细胞浓度的降低而提高。
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